Which is the best software to design cDNA primers and where is it available?

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Stefan Rödiger

There are a lot of tools. My favorite is PerlPrimer by Owen Mashall

http://perlprimer.sourceforge.net/

Marshall OJ. PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR. Bioinformatics 2004 20(15):2471-2472

Online resources:

http://openwetware.org/wiki/Choosing_primers_for_qPCR

http://www.gene-quantification.de/real-time-pcr-guide-bio-rad.pdf

In general you will find useful information at http://www.gene-quantification.de

Besides that you should consider to use primers from publications or at least refer to them.

Salman B. Hosain

You can use Vector NTI. It's very user friendly software.

http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/vector-nti-software.html?CID=fl-bioinformatics