I need a non-surgical method for early mouse embryos recovery (morula or blastocyst stage) such as uterine lavage.
I have not heard but idea is not bad for someone to try by some fine device to flush uteri. Still there will be one disadvantage if some morulas are on the way to uteri or some embryos remains unflushed, the mouse may become pregnant, anyway. The cost of housing for full term needs to be considered.
I have never done it but you might try with the NSET device that is used for non-surgical embryo transfer in mice.
Maybe you can try flushing the uterus with the NSET device but just for obtaining late preimplantation stage embryos (blastocysts). But you´ll need to flush a large amount of medium, and I don´t think that the female is going to stay quiet with this procedure...
I agree with you Gonzalo Moreno, but maybe if you use anesthesia you could obtain better results and also it should be easier to manipulate the females.
I'm also not aware of any non-invasive method to do this. By anaesthetising the female as suggested by Lessly there is the risk that this may impact on the metabolism of these early embryos. Any pharmacological intervention in the mother should be avoided.
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Disease Models
- Animal Models
- Rodent Models
- Mouse Models