I noticed in publication that lethal heat for cell line is 45 C for 60 min [Cell Stress & Chaperones (2001) 6 (4), 326–336].
Is this degree considered lethal for all cell lines?
Is there any species difference?
Your experience and contribution is appreciated.
Dear Colleagues,
I would like to share our findings regarding camel somatic cell thermotolerance to 45 C for 20h.
Article Differences between the tolerance of camel oocytes and cumul...
I think that may be due to species, the way of cell heating, and also moisture content in tissue or protein conformation. It need depth research.
Hello, Islam.
Obviously, there is species-specificity in thermotolerance as well as tissue-specific tolerance. We used to culture porcine follicular oocytes at 39C, avian spermatozoa at 40C, zebarfish embryos and fry at 28C. We also found different heat shock responses among different brain regions of zebrafish. So, above the tolerance would be lethal. There is no single absolute rule even in a species. Cell line cells are responding different species of HSPs depending on the temperature or duration. Sometimes, cells could be acclimated to the hyperthermic condition with longer and gradual manipulation of temperature.
Regards.
Dear Colleagues,
I would like to share our findings regarding camel somatic cell thermotolerance to 45 C for 20h.
Article Differences between the tolerance of camel oocytes and cumul...
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