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Questions related from Islam Saadeldin
What is the minimum number for biological and technical replicates for a reliable results of RT-qPCR? what if I collect/gather all the biological samples from different replicates of the same...
08 August 2018 420 5 View
Is there a method to detect trehalose in mammalian cell culture?
03 March 2018 3,075 4 View
I am culturing one kind of cells with some stem cells criteria. While I was preparing embryoid bodies in Petri dishes from these cells, they were attached to the bottom and grow naturally and now...
11 November 2017 2,282 3 View
I noticed in publication that lethal heat for cell line is 45 C for 60 min [Cell Stress & Chaperones (2001) 6 (4), 326–336]. Is this degree considered lethal for all cell lines? Is there any...
10 October 2017 2,150 5 View
Melatonin is derived from amino acid L-tryptophan. Is there any difference between ELISA specificity to the endogenous (naturally occurring) and the exogenous (drug or implants) melatonin?
08 August 2016 7,520 5 View
I need a non-surgical method for early mouse embryos recovery (morula or blastocyst stage) such as uterine lavage.
11 November 2015 229 7 View
This week another three papers have been retracted from Nature Cell Biology journal authored by S. Kato. Until now more than 25 papers have been retracted from highly prestigious journals...
11 November 2014 1,297 14 View
Chimeric embryo and/or animals can be generated through injection of pluripotent cells in the early stage developing embryos (Morula or blastocyst) or through aggregation of different kinds of...
11 November 2014 6,223 11 View
I want to isolate RNA and miRNA from a single cell. I read the work of Dr Wangh L.J. laboratory and I know some commercial kits but I need a cheap/easy method. I read an abstract from Applied...
12 December 2013 4,318 3 View
Microvesicles (MVs) or exosomes measured 30-1000 nm and all publications noted them through electron microscope but unfortunately EM is unavailable in our institute. Is it possible to visualize...
07 July 2013 3,733 9 View