Islam Saadeldin

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Questions related from Islam Saadeldin

What are the acceptable biological and technical replicates in realtime RT-qPCR?

What is the minimum number for biological and technical replicates for a reliable results of RT-qPCR? what if I collect/gather all the biological samples from different replicates of the same...

08 August 2018 401 5 View

How to detect trehalose in cultured cells?

Is there a method to detect trehalose in mammalian cell culture?

03 March 2018 3,058 4 View

How can I study cells that can attach to Petri dishes and grow very well?

I am culturing one kind of cells with some stem cells criteria. While I was preparing embryoid bodies in Petri dishes from these cells, they were attached to the bottom and grow naturally and now...

11 November 2017 2,267 3 View

What is the lethal heat for cell lines?

I noticed in publication that lethal heat for cell line is 45 C for 60 min [Cell Stress & Chaperones (2001) 6 (4), 326–336]. Is this degree considered lethal for all cell lines? Is there any...

10 October 2017 2,136 5 View

What is the specificity of ELISA in evaluating melatonin from different sources or different species?

Melatonin is derived from amino acid L-tryptophan. Is there any difference between ELISA specificity to the endogenous (naturally occurring) and the exogenous (drug or implants) melatonin?

08 August 2016 7,503 5 View

Is there any non-invasive (non-surgical) method for early mouse embryos retrieval ?

I need a non-surgical method for early mouse embryos recovery (morula or blastocyst stage) such as uterine lavage.

11 November 2015 215 7 View

The case of Shigeaki Kato, is not worrying for the future of science?

This week another three papers have been retracted from Nature Cell Biology journal authored by S. Kato. Until now more than 25 papers have been retracted from highly prestigious journals...

11 November 2014 1,281 14 View

What is the scientific importance of chimera/chimeric animals?

Chimeric embryo and/or animals can be generated through injection of pluripotent cells in the early stage developing embryos (Morula or blastocyst) or through aggregation of different kinds of...

11 November 2014 6,209 11 View

Can I isolate RNA and miRNA from a single cell by heating at 95 C?

I want to isolate RNA and miRNA from a single cell. I read the work of Dr Wangh L.J. laboratory and I know some commercial kits but I need a cheap/easy method. I read an abstract from Applied...

12 December 2013 4,301 3 View

Can I visualize the microvesicles or the exosomes by confocal or phase contrast microscopes?

Microvesicles (MVs) or exosomes measured 30-1000 nm and all publications noted them through electron microscope but unfortunately EM is unavailable in our institute. Is it possible to visualize...

07 July 2013 3,719 9 View