I want to isolate RNA and miRNA from a single cell. I read the work of Dr Wangh L.J. laboratory and I know some commercial kits but I need a cheap/easy method.
I read an abstract from Applied Biosystems (AB) in which they heated single cells at 95 C for 5 min (attached file). Does AB method work efficiently? If anyone has experience with this method please tell me in details.
Hi Islam,
Try using Norgen's Single Cell RNA Kit
http://norgenbiotek.com/display-product.php?ID=543
Norgen's kit uses a special patented resin (silicon carbide) that could bind large RNA and microRNA without the use of phenol. You will have to use phenol for all silica kits (Qiagen or Ambion) if you want to get microRNA. The problem with any kit using phenol is that there is always a chance (minimum 10%) of phenol carrier over that will completely inhibit your downstream applications.
Moreover, silica column usually requires carrier RNA at the single cell level. In contrast, silicon carbide has much better affinity and could bind very low amount of RNA as is. Without the use of carrier will make RNASeq at Single Cell level more enabling.
Contact the marketing team at Norgen ([email protected]). They may even be able to send you a sample kit to test.
Bernard
Thanks Andrei and Bernard for your helpful comments.
Best regards,
Islam
Dear colleagues,
I would like to share this new paper in "Cell" etiltles "Genome Analyses of Single Human Oocytes".
It linked to this topic.
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