Is there any good loading control for the analysis of secreted proteins to the media?

Gonzalo González Domínguez @Gonzalo-Gonzalez-Dominguez

04 June 2021 1 7K Report

Hi, we want to perform an experiment analyzing the supernatants. However, we don´t know which could be a suitable loading control. We have considered GADPH, fibronectin, or cyclophilin. Any suggestion?

Thank you!

Srinivas Kasulla

Gonzalo González Domínguez i guess similar type of question and discussion has already happened and am sure might be helpful for you

https://www.researchgate.net/post/How_to_normalize_protein_secreted_from_cell_culture_in_western_of_supernatants

Towards a better analysis of secreted proteins is what I felt a good research and targetted answer for your question and the link for the same is shared here with please have a look

Article Toward a better analysis of secreted proteins: The example o...

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