Gonzalo González Domínguez

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Questions related from Gonzalo González Domínguez

Is polybrene necessary for lentiviral titration with HEK293T?

Hi! We are producing lentivirus for T cell transduction. However, there are several protocols that do not mention the use of polybrene for titration in HEK293T, but they use centrifugation...

04 October 2023 7,314 2 View

Should I need to add Il-2 to a human CD3/CD4/CD8 T cell culture to induce exhaustion?

Hi! I want to induce an in vitro exhaustion model for human T cells, purified by magnetic columns. I found a protocol in which the authors cultivate the cells with Dyna beads (I have other...

16 August 2023 6,527 0 View

A suitable method or protocol for Indirect IF antibodies titration?

Hello everyone, we have recently acquired two antibodies: a polyclonal antibody against our antigen of interest, and it's secondary fluorescent conjugated. We transfect HEK293 T cells and then we...

19 May 2022 8,535 0 View

Has anybody performed an indirect staining for flow cytometry?

Hello! Besides through IF, we want to detect an intracellular recombinant protein through flow cytometry. However, we don´t have a conjugated antibody against it, so we plan to detect it through...

04 June 2021 7,506 2 View

Can i leave my WB mmbrane in PBS or Blocking buffer for 3 days at 4°C?

Hi everyone, i wonder if leaving my WB membrane in PBS or blocking buffer (milk 5% in PBS) will affect the results. I usually leave it on blocking buffer for an O.N. at 4 °C. Regrests, Gonzalo.

26 September 2019 1,869 1 View