8 Questions 5 Answers 0 Followers
Questions related from Gonzalo González Domínguez
Regarding CAR-T cell therapy, during co-cultures with different effector:target ratios, when we refer to "effector", does it involve the total T cells? For example, does a ratio of 5:1 mean 5...
29 May 2024 2,710 4 View
Hi! I was wondering if anyone has taken confocal images of apoptotic cells stained with Apotracker Tetra, from Biolegend. There is information using Apotracker Green, but I haven´t found a...
07 February 2024 8,319 0 View
Hello, has someone performed a T cell FACS Sorting after lentiviral transduction? We would like to purify those T cells that were transduced using GFP as reporter, and continue expanding the cells...
22 January 2024 2,512 0 View
Hi! We are producing lentivirus for T cell transduction. However, there are several protocols that do not mention the use of polybrene for titration in HEK293T, but they use centrifugation...
04 October 2023 7,331 2 View
Hi! I want to induce an in vitro exhaustion model for human T cells, purified by magnetic columns. I found a protocol in which the authors cultivate the cells with Dyna beads (I have other...
16 August 2023 6,545 0 View
Hello everyone, we have recently acquired two antibodies: a polyclonal antibody against our antigen of interest, and it's secondary fluorescent conjugated. We transfect HEK293 T cells and then we...
19 May 2022 8,544 0 View
Hello! Besides through IF, we want to detect an intracellular recombinant protein through flow cytometry. However, we don´t have a conjugated antibody against it, so we plan to detect it through...
04 June 2021 7,520 2 View
Hi everyone, i wonder if leaving my WB membrane in PBS or blocking buffer (milk 5% in PBS) will affect the results. I usually leave it on blocking buffer for an O.N. at 4 °C. Regrests, Gonzalo.
26 September 2019 1,885 1 View
