Is it possible to get intense signal using Cy3 filters for FAM-labeled probes?

My colleague and I did FISH using a FAM-labeled probe. We observed signals using FITC filters. However, when we tried to use the Cy3 filter, we could see intense and distinct signals not observed...

03 April 2017 8,759 3 View

How can I view the GC% of my DNA sequence in a graphical view?

Like I want to make a 100bp or 120bp sliding window GC% graph. Thank you!

08 September 2015 6,191 0 View

How can I compute sequence similarities using MEGA?

I have more than 10 sequences. I want to know the sequence similarity or identity of one sequence against each other sequences. How can I do that? Thank you.

11 December 2014 5,159 2 View

What online database should I use to BLAST my list of ~5-15 bp DNA sequences?

Hello. I have several short-length DNA sequences. I want to know which online database you recommend to know if my sequences match to known DNA patterns/motifs? Thanks a lot.

11 December 2014 8,186 4 View

How do I find my gene's UTR regions?

I found a gene in my plant sample through a homology search. I only got the CDS region though from our RNA database. How can I know the extent of the boundaries of the 5' and 3' regions of this...

08 September 2014 9,440 5 View

Why do I get the cloned insert size rather than the plasmid size when I gel analyze after maxiprep?

I did maxiprep plasmid/BAC isolation. When I did gel analyze, I was expecting for the classical two bands for the supercoiled and linear plasmids. Instead, I got real intense bands that...

06 July 2014 8,001 13 View

How can I estimate the GC content of a genome?

Is there any method that I can use on genomic DNA to estimate the GC content of a particular plant species' genome? I was wondering whether High Resolution Melt (HRM) could do it.

03 April 2014 2,996 12 View

Is there any laboratory that gives workshop of Fluorescence activated Chromosome Sorting (FACS)?

I was asked by my professor to isolate a single chromosome from my plant sample. I learned this could be done by flow karyotyping and then sorting using FACS. The thing is I don't know anyone in...

01 February 2013 8,759 3 View

What primer sequences are used to amplify telomeres of plants?

I want to amplify about 1 kb sequence of my plant sample. Is it possible using the repetitive telomere sequence?

11 December 2012 8,828 5 View

Can anyone help me with my ITS amplification?

Anyone working with the amplification of ITS1-5.8S-ITS2, how may bands do you usually get using primers that span from the 3'-end of 18S and the 5'-end of 26S? I got 2 bands from my sample and...

10 November 2012 1,076 12 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

No title

02 March 2021 3,060 3 View