Like I want to make a 100bp or 120bp sliding window GC% graph. Thank you!
My colleague and I did FISH using a FAM-labeled probe. We observed signals using FITC filters. However, when we tried to use the Cy3 filter, we could see intense and distinct signals not observed...
03 April 2017 8,759 3 View
I have more than 10 sequences. I want to know the sequence similarity or identity of one sequence against each other sequences. How can I do that? Thank you.
11 December 2014 5,159 2 View
Hello. I have several short-length DNA sequences. I want to know which online database you recommend to know if my sequences match to known DNA patterns/motifs? Thanks a lot.
11 December 2014 8,186 4 View
I found a gene in my plant sample through a homology search. I only got the CDS region though from our RNA database. How can I know the extent of the boundaries of the 5' and 3' regions of this...
08 September 2014 9,440 5 View
I did maxiprep plasmid/BAC isolation. When I did gel analyze, I was expecting for the classical two bands for the supercoiled and linear plasmids. Instead, I got real intense bands that...
06 July 2014 8,001 13 View
Is there any method that I can use on genomic DNA to estimate the GC content of a particular plant species' genome? I was wondering whether High Resolution Melt (HRM) could do it.
03 April 2014 2,996 12 View
I want to isolate single-stranded DNA from a mixture with double-stranded ones in a reassociation experiment. Any help will be greatly appreciated. Thank you!
31 December 2013 4,987 3 View
I was asked by my professor to isolate a single chromosome from my plant sample. I learned this could be done by flow karyotyping and then sorting using FACS. The thing is I don't know anyone in...
01 February 2013 8,759 3 View
I want to amplify about 1 kb sequence of my plant sample. Is it possible using the repetitive telomere sequence?
11 December 2012 8,828 5 View
Anyone working with the amplification of ITS1-5.8S-ITS2, how may bands do you usually get using primers that span from the 3'-end of 18S and the 5'-end of 26S? I got 2 bands from my sample and...
10 November 2012 1,076 12 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View