Is it possible to get intense signal using Cy3 filters for FAM-labeled probes?

My colleague and I did FISH using a FAM-labeled probe. We observed signals using FITC filters. However, when we tried to use the Cy3 filter, we could see intense and distinct signals not observed...

03 April 2017 8,759 3 View

How can I view the GC% of my DNA sequence in a graphical view?

Like I want to make a 100bp or 120bp sliding window GC% graph. Thank you!

08 September 2015 6,191 0 View

What online database should I use to BLAST my list of ~5-15 bp DNA sequences?

Hello. I have several short-length DNA sequences. I want to know which online database you recommend to know if my sequences match to known DNA patterns/motifs? Thanks a lot.

11 December 2014 8,186 4 View

How do I find my gene's UTR regions?

I found a gene in my plant sample through a homology search. I only got the CDS region though from our RNA database. How can I know the extent of the boundaries of the 5' and 3' regions of this...

08 September 2014 9,440 5 View

Why do I get the cloned insert size rather than the plasmid size when I gel analyze after maxiprep?

I did maxiprep plasmid/BAC isolation. When I did gel analyze, I was expecting for the classical two bands for the supercoiled and linear plasmids. Instead, I got real intense bands that...

06 July 2014 8,001 13 View

How can I estimate the GC content of a genome?

Is there any method that I can use on genomic DNA to estimate the GC content of a particular plant species' genome? I was wondering whether High Resolution Melt (HRM) could do it.

03 April 2014 2,996 12 View

Is there any enzyme that preferentially degrades double-stranded DNA and keeps single-stranded ones?

I want to isolate single-stranded DNA from a mixture with double-stranded ones in a reassociation experiment. Any help will be greatly appreciated. Thank you!

31 December 2013 4,987 3 View

Is there any laboratory that gives workshop of Fluorescence activated Chromosome Sorting (FACS)?

I was asked by my professor to isolate a single chromosome from my plant sample. I learned this could be done by flow karyotyping and then sorting using FACS. The thing is I don't know anyone in...

01 February 2013 8,759 3 View

What primer sequences are used to amplify telomeres of plants?

I want to amplify about 1 kb sequence of my plant sample. Is it possible using the repetitive telomere sequence?

11 December 2012 8,828 5 View

Can anyone help me with my ITS amplification?

Anyone working with the amplification of ITS1-5.8S-ITS2, how may bands do you usually get using primers that span from the 3'-end of 18S and the 5'-end of 26S? I got 2 bands from my sample and...

10 November 2012 1,076 12 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

The procedure compute?

02 March 2021 5,204 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View