Is it possible to get intense signal using Cy3 filters for FAM-labeled probes?

My colleague and I did FISH using a FAM-labeled probe. We observed signals using FITC filters. However, when we tried to use the Cy3 filter, we could see intense and distinct signals not observed...

03 April 2017 8,759 3 View

How can I view the GC% of my DNA sequence in a graphical view?

Like I want to make a 100bp or 120bp sliding window GC% graph. Thank you!

08 September 2015 6,191 0 View

How can I compute sequence similarities using MEGA?

I have more than 10 sequences. I want to know the sequence similarity or identity of one sequence against each other sequences. How can I do that? Thank you.

11 December 2014 5,159 2 View

How do I find my gene's UTR regions?

I found a gene in my plant sample through a homology search. I only got the CDS region though from our RNA database. How can I know the extent of the boundaries of the 5' and 3' regions of this...

08 September 2014 9,440 5 View

Why do I get the cloned insert size rather than the plasmid size when I gel analyze after maxiprep?

I did maxiprep plasmid/BAC isolation. When I did gel analyze, I was expecting for the classical two bands for the supercoiled and linear plasmids. Instead, I got real intense bands that...

06 July 2014 8,001 13 View

How can I estimate the GC content of a genome?

Is there any method that I can use on genomic DNA to estimate the GC content of a particular plant species' genome? I was wondering whether High Resolution Melt (HRM) could do it.

03 April 2014 2,996 12 View

Is there any enzyme that preferentially degrades double-stranded DNA and keeps single-stranded ones?

I want to isolate single-stranded DNA from a mixture with double-stranded ones in a reassociation experiment. Any help will be greatly appreciated. Thank you!

31 December 2013 4,987 3 View

Is there any laboratory that gives workshop of Fluorescence activated Chromosome Sorting (FACS)?

I was asked by my professor to isolate a single chromosome from my plant sample. I learned this could be done by flow karyotyping and then sorting using FACS. The thing is I don't know anyone in...

01 February 2013 8,759 3 View

What primer sequences are used to amplify telomeres of plants?

I want to amplify about 1 kb sequence of my plant sample. Is it possible using the repetitive telomere sequence?

11 December 2012 8,828 5 View

Can anyone help me with my ITS amplification?

Anyone working with the amplification of ITS1-5.8S-ITS2, how may bands do you usually get using primers that span from the 3'-end of 18S and the 5'-end of 26S? I got 2 bands from my sample and...

10 November 2012 1,076 12 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View