Is there any protein which is functionally similar to GnIH?

I am working on catfish model (Clarias magur). I have done transcriptome analysis of the brain tissue of this fish. But, no transcript of gnih has been detected. I wanted to know, whether any...

10 November 2018 5,246 0 View

Can anyone help me the protocol for the pituitary culture of fish?

I want to do the in vitro culture of the fish pituitary under different treatment condition. So, can anyone kindly help me the protocol for the pituitary culture. Thanks,

08 September 2018 794 2 View

What is the difference between Life Science and Biological Science?

Is there any major difference between the Ph.D. in Life Science and Ph.D. in Biological Science?

07 August 2018 9,454 2 View

What happen if acrophase value was recorded more than 24h in the single cosinor analysis in fish model for particular genes expression?

02 March 2018 5,022 1 View

How much affects on the circadian clock gene expression due to different colors of light on teleosts?

08 September 2016 583 2 View

Is it necessary to have full length sequence of mRNA for Real-time PCR?

I have checked mRNA expression pattern of certain genes using partial sequence. I have communicated a paper with the expression result. Reviewer has asked, why have I taken partial sequence...

05 June 2016 3,591 3 View

How to perform primary pinealocytes culture from the fish species?

I want to do the primary pinealocytes culture from the fish pineal tissue. So I need the protocol for this culture, anyone kindly suggest me any good protocol or any research article related to...

03 April 2016 4,819 4 View

Does continuous light (LL) and continuous darkness (DD) affect the circadian rhythm of clock genes expression on fish pineal at 24 hours time period?

I wanted to know any affect of continuous light (LL) and continuous darkness (DD) on the circadian rhythm of clock genes expression on fish pineal at 24 hours time period, mainly clock and bmal...

31 December 2015 7,099 4 View

How to calculate goodness of fit?

Can anyone kindly suggest me any statistical software of statistical tools which one help me to analysis the goodness of fit.

31 December 2015 2,750 12 View

Does among the all fishes showed same clock gene expression pattern due to particular photoperiod condition or different different patterns?

Due to different photoperiod such as under 12L-12D, short day, long day, LL and DD condition the fishes were showed different clock gene expression on particular fish pineal. I am working on...

31 December 2015 7,825 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View