Is there any protein which is functionally similar to GnIH?

I am working on catfish model (Clarias magur). I have done transcriptome analysis of the brain tissue of this fish. But, no transcript of gnih has been detected. I wanted to know, whether any...

10 November 2018 5,246 0 View

Can anyone help me the protocol for the pituitary culture of fish?

I want to do the in vitro culture of the fish pituitary under different treatment condition. So, can anyone kindly help me the protocol for the pituitary culture. Thanks,

08 September 2018 794 2 View

What is the difference between Life Science and Biological Science?

Is there any major difference between the Ph.D. in Life Science and Ph.D. in Biological Science?

07 August 2018 9,454 2 View

What happen if acrophase value was recorded more than 24h in the single cosinor analysis in fish model for particular genes expression?

02 March 2018 5,022 1 View

How much affects on the circadian clock gene expression due to different colors of light on teleosts?

08 September 2016 583 2 View

Is it necessary to have full length sequence of mRNA for Real-time PCR?

I have checked mRNA expression pattern of certain genes using partial sequence. I have communicated a paper with the expression result. Reviewer has asked, why have I taken partial sequence...

05 June 2016 3,591 3 View

Does continuous light (LL) and continuous darkness (DD) affect the circadian rhythm of clock genes expression on fish pineal at 24 hours time period?

I wanted to know any affect of continuous light (LL) and continuous darkness (DD) on the circadian rhythm of clock genes expression on fish pineal at 24 hours time period, mainly clock and bmal...

31 December 2015 7,099 4 View

How to calculate goodness of fit?

Can anyone kindly suggest me any statistical software of statistical tools which one help me to analysis the goodness of fit.

31 December 2015 2,750 12 View

Is there any change of circadian rhythm of clock genes on fish pineal due to different seasons at 24 h time period?

31 December 2015 6,858 4 View

Does among the all fishes showed same clock gene expression pattern due to particular photoperiod condition or different different patterns?

Due to different photoperiod such as under 12L-12D, short day, long day, LL and DD condition the fishes were showed different clock gene expression on particular fish pineal. I am working on...

31 December 2015 7,825 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View