Erik Maronde

6 Questions 103 Answers 0 Followers

Questions related from Erik Maronde

Can someone tell me which insect this is (I am no insect specialist)?

I made this foto in our garden last week. Can someone tell me which insect this is (I am no insect specialist)?

06 June 2016 3,486 6 View

Can someone recommend a mouse vasoactive intestinal peptide (VIP) ELISA for cell culture supernatants?

I work in a cell line expressing and storing VIP and look for a robust and sensitive ELISA for VIP in the supernatants (serum-free). Can someone recommend an assay, no matter if commercial or...

06 June 2015 4,531 2 View

Is mouse hippocampal neuronal differentiation in vitro comparable to in vivo?

Many groups use newborn mouse hippocampal cell preparations to analyse differentiation and signaling processes. These cultures are usually kept cultivated for 2 to 4 weeks in vitro before use. Do...

10 October 2014 8,265 6 View

Is it time to have a resource for biological knowledge and understanding like the Beilstein is for Chemistry?

New papers on biological principles and mechanisms appear on a daily basis. May it be necessary or useful to attempt an approach like the Beilstein for Chemicals and Chemistry on a "ten years...

05 May 2014 1,513 2 View

Are there specific or selective activators and/or inhibitors for cyclic nucleotide gated ion channels for use in a cell-culture system?

I observe a clear effect with forskolin and cyclic nucleotide-dependent protein kinase activators (Sp-cAMPS, SpcDDBIMPS), but cannot inhibit that with myr-PKI. The EPAC-specific agonist has no...

07 July 2013 6,552 24 View

Is myr-PKI a good PKA inhibitor in cell culture?

I treat cells with forskolin and measure CRE-luc reporter gene activity (which is elevated 30 to 50 fold by 10 µM forskolin). Rp-cAMPS and 8Br-Rp-cAMPS (both 1 mM) inhibit this nicely. Myr-PKI...

09 September 2012 8,066 13 View