How much affects on the circadian clock gene expression due to different colors of light on teleosts?

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Is there any protein which is functionally similar to GnIH?

I am working on catfish model (Clarias magur). I have done transcriptome analysis of the brain tissue of this fish. But, no transcript of gnih has been detected. I wanted to know, whether any...

10 November 2018 5,421 0 View

Can anyone help me the protocol for the pituitary culture of fish?

I want to do the in vitro culture of the fish pituitary under different treatment condition. So, can anyone kindly help me the protocol for the pituitary culture. Thanks,

08 September 2018 974 2 View

What is the difference between Life Science and Biological Science?

Is there any major difference between the Ph.D. in Life Science and Ph.D. in Biological Science?

07 August 2018 9,912 2 View

What happen if acrophase value was recorded more than 24h in the single cosinor analysis in fish model for particular genes expression?

The acrophase means 24h cycle. If the acrophase value came more than 24h assume, 25.78 ± 1.78 in cosinor analysis using the formula. f(t) =A+ (B * Cos [2 * pi...

02 March 2018 5,218 1 View

Is it necessary to have full length sequence of mRNA for Real-time PCR?

I have checked mRNA expression pattern of certain genes using partial sequence. I have communicated a paper with the expression result. Reviewer has asked, why have I taken partial sequence...

05 June 2016 3,755 3 View

How to perform primary pinealocytes culture from the fish species?

I want to do the primary pinealocytes culture from the fish pineal tissue. So I need the protocol for this culture, anyone kindly suggest me any good protocol or any research article related to...

03 April 2016 5,019 4 View

Does continuous light (LL) and continuous darkness (DD) affect the circadian rhythm of clock genes expression on fish pineal at 24 hours time period?

I wanted to know any affect of continuous light (LL) and continuous darkness (DD) on the circadian rhythm of clock genes expression on fish pineal at 24 hours time period, mainly clock and bmal...

31 December 2015 7,288 4 View

How to calculate goodness of fit?

Can anyone kindly suggest me any statistical software of statistical tools which one help me to analysis the goodness of fit.

31 December 2015 2,951 12 View

Does among the all fishes showed same clock gene expression pattern due to particular photoperiod condition or different different patterns?

Due to different photoperiod such as under 12L-12D, short day, long day, LL and DD condition the fishes were showed different clock gene expression on particular fish pineal. I am working on...

31 December 2015 8,007 3 View

Is there any change of circadian rhythm of clock genes on fish pineal due to different seasons at 24 h time period?

Due to different seasons like summer, winter and spring how much changes the circadian rhythm of clock genes expression on fish pineal?

31 December 2015 7,056 4 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

While doing Chronoamperometry, when the light is turned off, why the current is not decreasing sharply?

Here, I have used Ag/AgCl as reference electrode and 0.5M KOH.

29 July 2024 4,538 3 View