Can anyone help me the protocol for the pituitary culture of fish?

I want to do the in vitro culture of the fish pituitary under different treatment condition. So, can anyone kindly help me the protocol for the pituitary culture. Thanks,

08 September 2018 794 2 View

What is the difference between Life Science and Biological Science?

Is there any major difference between the Ph.D. in Life Science and Ph.D. in Biological Science?

07 August 2018 9,454 2 View

What happen if acrophase value was recorded more than 24h in the single cosinor analysis in fish model for particular genes expression?

02 March 2018 5,022 1 View

How much affects on the circadian clock gene expression due to different colors of light on teleosts?

08 September 2016 583 2 View

Is it necessary to have full length sequence of mRNA for Real-time PCR?

I have checked mRNA expression pattern of certain genes using partial sequence. I have communicated a paper with the expression result. Reviewer has asked, why have I taken partial sequence...

05 June 2016 3,591 3 View

How to perform primary pinealocytes culture from the fish species?

I want to do the primary pinealocytes culture from the fish pineal tissue. So I need the protocol for this culture, anyone kindly suggest me any good protocol or any research article related to...

03 April 2016 4,819 4 View

Does continuous light (LL) and continuous darkness (DD) affect the circadian rhythm of clock genes expression on fish pineal at 24 hours time period?

I wanted to know any affect of continuous light (LL) and continuous darkness (DD) on the circadian rhythm of clock genes expression on fish pineal at 24 hours time period, mainly clock and bmal...

31 December 2015 7,099 4 View

How to calculate goodness of fit?

Can anyone kindly suggest me any statistical software of statistical tools which one help me to analysis the goodness of fit.

31 December 2015 2,750 12 View

Is there any change of circadian rhythm of clock genes on fish pineal due to different seasons at 24 h time period?

31 December 2015 6,858 4 View

Does among the all fishes showed same clock gene expression pattern due to particular photoperiod condition or different different patterns?

Due to different photoperiod such as under 12L-12D, short day, long day, LL and DD condition the fishes were showed different clock gene expression on particular fish pineal. I am working on...

31 December 2015 7,825 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View