I am working on catfish model (Clarias magur). I have done transcriptome analysis of the brain tissue of this fish. But, no transcript of gnih has been detected. I wanted to know, whether any functionally similar to GnIH protein is there or not.
I want to do the in vitro culture of the fish pituitary under different treatment condition. So, can anyone kindly help me the protocol for the pituitary culture. Thanks,
08 September 2018 974 2 View
Is there any major difference between the Ph.D. in Life Science and Ph.D. in Biological Science?
07 August 2018 9,912 2 View
The acrophase means 24h cycle. If the acrophase value came more than 24h assume, 25.78 ± 1.78 in cosinor analysis using the formula. f(t) =A+ (B * Cos [2 * pi...
02 March 2018 5,218 1 View
Is there any type of circadian clock rhythmic expression happens due to different colors of light? Or different intensity of the light can affects? Kindly give me any suggestion. Thanks
08 September 2016 750 2 View
I have checked mRNA expression pattern of certain genes using partial sequence. I have communicated a paper with the expression result. Reviewer has asked, why have I taken partial sequence...
05 June 2016 3,755 3 View
I want to do the primary pinealocytes culture from the fish pineal tissue. So I need the protocol for this culture, anyone kindly suggest me any good protocol or any research article related to...
03 April 2016 5,019 4 View
I wanted to know any affect of continuous light (LL) and continuous darkness (DD) on the circadian rhythm of clock genes expression on fish pineal at 24 hours time period, mainly clock and bmal...
31 December 2015 7,288 4 View
Can anyone kindly suggest me any statistical software of statistical tools which one help me to analysis the goodness of fit.
31 December 2015 2,951 12 View
Due to different photoperiod such as under 12L-12D, short day, long day, LL and DD condition the fishes were showed different clock gene expression on particular fish pineal. I am working on...
31 December 2015 8,007 3 View
Due to different seasons like summer, winter and spring how much changes the circadian rhythm of clock genes expression on fish pineal?
31 December 2015 7,056 4 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View