I want to do the in vitro culture of the fish pituitary under different treatment condition. So, can anyone kindly help me the protocol for the pituitary culture.
So far I remember, before going to the in vitro culture of the pituitary under different treatment conditions, you should think of the pituitary gland whether to collect it from the wild or cultivated; from carnivorous, omnivorous or herbivorous; from riverine or marine; or from coldwater or warm water?