I am trying to test out a mash1 reporter construct and have run into the issue that I don't have a proper cell line to test it out on. In the past, I have tried using mouse es cells and forcing neuronal differentiation to see mash1 expression, however it's both extremely time consuming and difficult to culture these cells.
I have also experimented with glioblastoma cell lines, as they express huge amounts of mash1, but they are partially adherent and partially free-floating, which makes drug selection extremely difficult to do.
Does anyone know of an adherent cell line which either stably or can be induced to express mash1?
Extra points if they are easily obtainable :D
Thanks!
In my experience with glioblastoma, if you culture them on matrigel-coated plates, they adhere 100%. Maybe that's your best option
There are several neural stem cell lines that express Ascl1 such as the NS5 line from Austin Smith's lab (see Conti et al., 2005 PLoS Biol). They will grow adherently on TC plastic (or gelatin or laminin-coated plastic).
A good option could be the rat phaeochromocytoma cell line PC12 (see for example Issack and Ziff, Cell growth Diif, 1998). They grow relatively well on plastic and they should not be too difficult to get hold of.
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