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Questions related from Brian Lin
Hi All, I'm wondering if anyone has tried out HCR in-situs out there, as I've found these very promising papers, and a company from the original lab that published them, but no other references to...
01 January 2018 9,783 13 View
It is my understanding that you don't add a polyA in lentiviral vectors, as it is contained within the LTR. However, in some circumstances, there is no obvious polyA in simple plasmids which are...
02 February 2014 9,286 13 View
A rather naive question incoming: is there a difference in efficacy between free base and citrate salt forms of tamoxifen? They have different solubilities according to sigma and some other sites,...
02 February 2014 4,071 2 View
I am doing practice runs of an ES cell lysis protocol that I am planning on using for southern blot analysis. I get the standard spidery precipitated DNA after addition of isopropanol, I spin it...
11 November 2013 4,055 6 View
I am trying to test out a mash1 reporter construct and have run into the issue that I don't have a proper cell line to test it out on. In the past, I have tried using mouse es cells and forcing...
07 July 2013 1,476 5 View
Short question: Suggestions for a purification method for a 20 kb plasmid that will give near-quantitative yield of the original input that is low salt? --- Long explanation: I'm interested in a...
04 April 2013 7,779 13 View
I'm trying to clone a 20 kb mouse targeting vector and it's giving me quite a hard time. I was wondering about two things: First, does anyone know of a particularly stable plasmid vector that...
01 January 2013 5,190 13 View
I'm trying to PCR a 4kb stretch of mouse genomic dna from a BAC prep using accuprime or phusion. In my first PCR, I will get a relatively low yielding product, with much more smaller off-target...
05 May 2012 7,756 36 View
