Hi experts,
I wonder if there is a possibility to seal well plates for cell culture in a sterile environment in way that liquids can remain in the well plates? Something like vacuum packaging where the lid is packed separately?
We have a hydrogel which should not dry out and ideally is immersed in buffer all the time. Now, we cannot transport the well plates far, since there's leaking of the liquid from the plates if tilted.
Sterility must be maintained.
Thank you very much!
Have you tried parafilm? If applied correctly you should not have any leaks and it offers sterility
Evangelina, Thank you very much for your answer. I guess parafilm will not be stable enough and cannot be removed completely after a while when kept on the plastic. Also, can parafilm be autoclaved?
Marcus
I have been using parafilm to seal microplates and dishes with liquid inside /also for hydrogel(beads). I used to UV the parafilm strips inside the laminar flow cabinet and had no contamination issues. However sometimes they stick to the plates as you mentioned. Maybe look into microplate adhesive seals? There are many types and can be breathable
Hi Marcus,
my first guess would also have been parafilm + the lid of the dish.
However, one question would be what size your plates have and how far you need to transport/how long you need to store them. Sealing small wells with 2 layers of parafilm, fixing the parafilm afterwards with the lid and storing in the fridge worked well for me.
I used to use parafilm as sealing a lot for flow chambers and had never any issues with sterility (sprayed it with EtOH and dried in the flow cabinet).
Katrin
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