Marcus Binner

9 Questions 169 Answers 0 Followers

Questions related from Marcus Binner

Is there a way to seal sterile cell culture plates and dishes with liquid in the wells?

Hi experts, I wonder if there is a possibility to seal well plates for cell culture in a sterile environment in way that liquids can remain in the well plates? Something like vacuum packaging...

09 September 2019 5,490 4 View

[solved] Can you explain a +44 mass difference in peptide synthesis?

Dear experts, after peptide synthesis (standard Fmoc chemistry, no acetylation between the couplings), I have a significant peak next to my main product for analytical reverse phase HPLC (see...

12 December 2018 3,252 7 View

Can you help me finding a list of transparent polymers suitable for light microscopy including refractive index?

We develop medical devices for characterization of thin tissues which should be produced from plastic/polymers (wall thickness

09 September 2018 9,830 8 View

Is there a formula/way to estimate the volume of polymers in solution with different architecture, but the same molecular weight?

Hi polymer experts, in our certain case, we analyzed poly(ethylene glycol) (PEG) with high performance size exclusion chromatography (HPSEC). Solvent was 10x PBS (aqueous solution). For instance,...

11 November 2016 1,664 6 View

Any advantages of Agilent G1379 1260 Micro Degasser towards 'normal' G1322 1200 or G1322A 1100 Degasser for analytical HPLC??

Hi experts, we currently struggle with our ~ 8-10 year old Agilent 1100 G1322A Degasser (I asked a question about 1.25 years ago). It worked very well and now the status LED is turning red - I...

07 July 2016 6,960 10 View

How to determine oxygen amount / composition of a gas sample?

Hi, we recently had problems with some syntheses where we use nitrogen flow to remove a byproduct (tBu-SH) from a reduction reaction from a reaction mixture. The educt is a StBu-protected peptide...

01 January 2016 2,949 5 View

[solved] How can I fix the issue of injected volumes not being right with an Agilent G1329A 1100 Series Autosampler?

Dear HPLC experts, we currently have rather huge problems with our G1329A autosampler - it does not constantly inject the right volume (which is crucial for quantitative analysis). For example, if...

12 December 2015 5,955 18 View

[solved] How to fix or reconstruct Q-TOF Tune Report from Agilent MassHunter workstation version B.05.01?

We had a computer crash and re-installed the Agilent MassHunter Software Version B.05.01, build 5.01.5215.1 back on the PC with new hard disks etc. with Agilent recovery CDs as...

10 October 2015 8,274 3 View

[solved] How can I decontaminate Agilent 1100 G1322A Degasser?

We do HPSEC with PBS buffer on a regular basis on our Agilent 1100 HPLC unit. One solvent line (A2) is in contact with PBS all the time. This morning I recognized a white coating inside the A2...

04 April 2015 8,610 13 View