What public open software would you recommend for designing HRM primers?

What public open software would you recommend for designing HRM primers?

12 October 2020 3,925 4 View

ISH or immunostaining of GABA neurons?

Hi, Can I reliably label GABA neurons using ISH instead immuno-staining? It is extremely difficult to stain GABA neurons in VLPO and MnPO of hypothalamus by antibody and it seems that ISH is more...

21 September 2020 2,503 4 View

Are there any approved products that can prevent or treat COVID-19 in animals?

10 September 2020 8,643 19 View

Are my birds safe from COVID-19, and is my family safe around my birds?

07 September 2020 4,102 17 View

What tests can be used for a random number generator besides diehard?

What tests can be used for a random number generator besides diehard? Need find tests for my random number generator, based on human-computer interaction.

15 May 2020 7,167 7 View

What is the average depth explored in metal oxide samples through the following characterization techniques: XRD, XPS and Raman spectroscopy?

Obviously, the exact depth evaluated by each characterization technique mentioned (XRD, XPS and Raman spectroscopy) depends on the specific parameters adopted for each analysis. However, in...

15 May 2020 6,418 16 View

How can I add the journal name if your system does not find it in the list?

Hi, I have the published papers (with DOI) in the academic journals that have official ISSN etc. But I can not add and save the names of the journals in my records because your system does not...

26 April 2020 9,394 5 View

What are the storage conditions for phosphines for Staudinger ligation?

Hi all, We would like to purchase 5 mg of phosphine-modified fluorescent dye for site-specific labeling of proteins containing the unnatural aminoacid azide-phenylalanine. We will be labeling...

05 March 2020 3,007 3 View

Metallic materials (more specifically, nickel) present characteristic peaks in Raman spectroscopy analysis?

When evaluating an alumina sample using Raman spectroscopy, characteristic peaks were obtained for certain wavelengths. However, after depositing a thin nickel film via plasma on the surface of...

24 January 2020 8,954 3 View

Does anybody have PDF version of MOE manual?

I will highly appreciate if you can share one for program version 2014 or higher.

28 May 2019 5,615 5 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View