What public open software would you recommend for designing HRM primers?

What public open software would you recommend for designing HRM primers?

12 October 2020 3,925 4 View

ISH or immunostaining of GABA neurons?

Hi, Can I reliably label GABA neurons using ISH instead immuno-staining? It is extremely difficult to stain GABA neurons in VLPO and MnPO of hypothalamus by antibody and it seems that ISH is more...

21 September 2020 2,503 4 View

Are there any approved products that can prevent or treat COVID-19 in animals?

10 September 2020 8,643 19 View

Are my birds safe from COVID-19, and is my family safe around my birds?

07 September 2020 4,102 17 View

What tests can be used for a random number generator besides diehard?

What tests can be used for a random number generator besides diehard? Need find tests for my random number generator, based on human-computer interaction.

15 May 2020 7,167 7 View

What is the average depth explored in metal oxide samples through the following characterization techniques: XRD, XPS and Raman spectroscopy?

Obviously, the exact depth evaluated by each characterization technique mentioned (XRD, XPS and Raman spectroscopy) depends on the specific parameters adopted for each analysis. However, in...

15 May 2020 6,418 16 View

How can I add the journal name if your system does not find it in the list?

Hi, I have the published papers (with DOI) in the academic journals that have official ISSN etc. But I can not add and save the names of the journals in my records because your system does not...

26 April 2020 9,394 5 View

What are the storage conditions for phosphines for Staudinger ligation?

Hi all, We would like to purchase 5 mg of phosphine-modified fluorescent dye for site-specific labeling of proteins containing the unnatural aminoacid azide-phenylalanine. We will be labeling...

05 March 2020 3,007 3 View

Metallic materials (more specifically, nickel) present characteristic peaks in Raman spectroscopy analysis?

When evaluating an alumina sample using Raman spectroscopy, characteristic peaks were obtained for certain wavelengths. However, after depositing a thin nickel film via plasma on the surface of...

24 January 2020 8,954 3 View

Does anybody have PDF version of MOE manual?

I will highly appreciate if you can share one for program version 2014 or higher.

28 May 2019 5,615 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View