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Questions related from Andrey Revyakin
I need to build a low-cost airflow humidifier which would not have a direct contact between water and the air to be humidified (i.e. not a bubbler or sponge). The "state of the art" on this are...
02 September 2023 4,920 1 View
What conditions do you use when cleaving DDE-based linkers in protein bio-conjugates (time, temperature, hydrazine concentration?) Are there linkers which can be cleaved in milder conditions...
14 November 2021 9,932 1 View
Is anyone familiar with DIY repair of the Typhoon (Amersham/GE/molecular dynamics) lasers canners? Can't find any hints online. Our current issue with it is a faulty shutter cable. BTW, in case...
28 September 2020 6,832 5 View
Hi all, We would like to purchase 5 mg of phosphine-modified fluorescent dye for site-specific labeling of proteins containing the unnatural aminoacid azide-phenylalanine. We will be labeling...
06 March 2020 3,256 3 View
Can anyone recommend a front-loading tabletop autoclave which could be used to sterilize up to six 2L flasks at once with steam? Looking for something that is robust, simple, and ages well (i.e....
01 August 2018 6,337 0 View
FBS-free medium is what I am looking for. If defined growth factors are required, I can express them (if they are available in recombinant form). Artificial meat companies seem to have figured it...
06 July 2018 1,556 3 View
I tried using a small amount of qiagen tip 20 resin in batch-mode for PCR product purification (equilibrate-bind-wash-elute, can't use silica spin columns for various reasons). Binding was near...
10 March 2018 5,233 2 View
We are designing gRNAs for targeting essential genes in human cells, and finding that common online tools give very inconsistent scores for same gRNA sequences (e.g. a gRNA scored at 26/100 by one...
30 March 2017 3,017 1 View
Does anyone have experience with the Misonix/QSonica sonicator throwing an 'out of lock' error . Power reads 0W as well, and no ultrasound comes out (even initially). Some initial horn check...
09 January 2017 972 4 View
I am looking to buy a simple PCR cycler. Was considering OpenPCR, but their web site seems to not have been updated for a while, and they do not return emails. Does anyone have experience with...
31 October 2016 8,501 0 View
For example, is it identical to NEB's phusion? The latter is described in: http://barricklab.org/twiki/bin/view/Lab/ProtocolsReagentsPhusion
01 October 2016 3,230 2 View
Hi all, I am trying to concentrate a DNA sample using dialysis against 50% PEG, through a 10K cut-off membrane. I made a 50% solution of PEG20K supplemented with 10mM Tris (target pH=7.8), and...
02 September 2016 8,405 9 View
I've been wondering whether there are any new manufacturers around who make reliable, affordable PCR machines. The Chinese now make great smartphones, may be they now make good cyclers, too...
12 March 2016 3,574 4 View
