Andrey Revyakin

5 Questions 24 Answers 0 Followers

Questions related from Andrey Revyakin

What are the storage conditions for phosphines for Staudinger ligation?

Hi all, We would like to purchase 5 mg of phosphine-modified fluorescent dye for site-specific labeling of proteins containing the unnatural aminoacid azide-phenylalanine. We will be labeling...

06 March 2020 3,134 3 View

Is there a chemically defined, FBS-free media formulation for HCT-116 and U2OS cells (adherent human cells)?

FBS-free medium is what I am looking for. If defined growth factors are required, I can express them (if they are available in recombinant form). Artificial meat companies seem to have figured it...

06 July 2018 1,480 3 View

Does anyone Happen to know what BioRad's iProof PCR enzyme is (beyond what's in the catalog description)?

For example, is it identical to NEB's phusion? The latter is described in: http://barricklab.org/twiki/bin/view/Lab/ProtocolsReagentsPhusion

01 October 2016 3,169 2 View

How to control pH and composition of PEG solutions?

Hi all, I am trying to concentrate a DNA sample using dialysis against 50% PEG, through a 10K cut-off membrane. I made a 50% solution of PEG20K supplemented with 10mM Tris (target pH=7.8), and...

02 September 2016 8,340 9 View

An affordable and reliable thermal cycler?

I've been wondering whether there are any new manufacturers around who make reliable, affordable PCR machines. The Chinese now make great smartphones, may be they now make good cyclers, too...

12 March 2016 3,434 4 View