Has annyone try to reuse EveryBlot Blocking Buffer (Bio-rad) for fluorescence western blotting?

Hello to every one, Our lab has recently switched our western blot technique from chemiluminescence to fluorescence. Therefore, we have to optimize the blocking buffer. We have successfully try...

05 February 2021 7,886 1 View

Does anyone know how to get a scopus or sciencedirect report of the trends in recent years on a specific research topic?

I am trying to obtain a trend report on a specific issue at your instigation in recent years

28 December 2020 848 2 View

Can the content of my research section be shown on the front page?

no details needed

26 December 2020 9,690 3 View

How do you transmit and evaluate ethics and moral values in you teaching activity?

Ethics and moral values have always being an essential part of teaching and scientific research. How do you teach and evaluate these essential subjects in your daily interaction with students?

23 December 2020 5,058 7 View

Can I use the images of Collection 1 Level 2 Landsat 5 TM / Landsat 8 OLI to perform vegetation indices right away?

I'm aware that in order to perform any vegetation index like NDVI, SAVI or OSAVI you need to do some atmospheric, radiometric and terrain corrections. I'm pretty sure that Level 2 images from...

21 December 2020 4,088 3 View

Does anyone know of cases of complete eye evulsion (eyes jumping out of their sockets) in ancient or medieval sources?

01 November 2020 1,234 3 View

Do you believe SARS-Cov-2 vaccines will yield a robust immune response and memory?

In view of the potential for Covid-19 reinfection and the weak immune response and short duration in patients previously infected with Covid-19, many doubts have emerged if the virus is capable of...

03 October 2020 5,689 7 View

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I'm currently working in a project in which it would be useful to measure: transmural flux, resistence and potencial difference in mucosa biopsies. I've been reading about this method and as far...

23 September 2020 6,976 1 View

mScarlet Elisa Detection?

I've been trying to detect mScarlet protein purified from E. coli via an ELISA assay. My protein has two tags: an N-terminal Strep-tag and a C-terminal His-tag. To do the ELISA I've used...

20 September 2020 730 2 View

No title

05 August 2020 8,976 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

How to apply a voltage sinusoidal/harmonic load in ANSYS APDL?

Hello all, I want to simulate a Dielectric Elastomer Actuator DAE (an electrostatic-Structural analysis). I want to apply various voltages and frequencies into the DEA in the form of sine wave....

13 February 2021 3,261 3 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View

How do I stop losing peak shape with Waters T3 Atlantis Column?

I am using this column with a gradient of acetonitrile vs a phosphate buffer. I clean the column after every run with 2%ACN:98%H20 and store in 30% ACN. I've noticed that my peaks are getting...

10 February 2021 9,181 1 View

How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

Handheld Portable Raman spectroscopy systems - recommendations and experience?

Hi all, I am looking for some general feedback and information from those that have experience using handheld/portable systems? I am looking at the prospects of using an off the shelf system from...

07 February 2021 9,691 3 View

How to clean up an already pure protein from grwoth medium?

Hi all, I have got a protein, expressed in P. pastoris vector, more than 97% (SDS-page) pure in the culture medium after cells separation. Despite the protein expressed has a his-tag, Nichel...

04 February 2021 5,173 6 View

Silane solution and application to Glass ?

30 January 2021 7,924 3 View