Hi there,
We are driving a project on breast cancer bearing female BALB/c mice. After 4 weeks interventions, 4T1 breast cancer carcinoma cell lines (650000-750000) were subcutaneously injected in the inguinal flank area near to back of animals. ""After 6-8 days cancer were palpable in the cells injection position of more than 85% of BALB/c mice""!!! Unfortunately, all mice have begun rejection of the tumors in the second and third weeks after injection!?!?! Now, less than 10% mice have tumor and the other ones (including intervention groups and control) have rejected the tumors!!! I don't know the main reason for this challenge! Is it possible that BALAB/c mice are not homozygous and they are heterozygous? Or it is because of technical error in tumor cell lines injection? Please advise as necessary.
Thanks!
Hi Mahmoud,
In my lab, we use 4T1 tumor cells. We usually implant 50K cells with marigel into 4th mammary fat pad and they grow well (our papers is attached).
Do your cells have GFP? GFP expressing tumors are rejected in Balb/c mice. In a recent paper published by Weinberg groups, they showed that GFP expressing tumors are started to shrinkage after 15-20 days in BALB/c mice. Check attached paper.
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