Does anyone have detailed methods and instructions on how to count the numbers of intraepidermal nerve fibers (IENFs)/mm using Fiji - ImgaeJ?

06 May 2024 1 3K Report

Counting the numbers of intraepidermal nerve fibers

Intraepidermal nerve fibers (IENFs) play a crucial role in diagnosing peripheral neuropathies. These small nerve fibers are located within the epidermis of the skin and can provide valuable information about disease progression, tissue responses to treatments, and the pathogenesis of peripheral nervous system disorders1.

Here are some key points about IENFs:

Purpose and Importance:Skin biopsies, specifically analyzing IENFs, have become an essential diagnostic tool for neurologists. Reduction in epidermal nerve density is a common neuropathologic abnormality observed in skin biopsies. Assessing changes in the morphology and density of IENFs helps diagnose and predict peripheral neuropathies. It also provides insights into disease progression and tissue responses to regenerative treatments.

Counting Techniques: Two counting methods have evolved: Counting Across Epidermal Basement Membrane:This method involves counting only the nerve fibers that cross the epidermal basement membrane. It provides a quantitative measure of IENFs. Including Isolated Nerve Fragments:This approach includes isolated nerve fragments within the epidermis, even if they do not cross the basement membrane. Both methods offer valuable diagnostic information1.

Immunohistochemical Analysis:Epidermal nerve fiber density testing involves immunohistochemical analysis of a punch biopsy of skin. It quantifies the number of unmyelinated C-fibers and myelinated A delta-fibers within the epidermis per unit area2.

Visualizing IENFs:Using an immunostain against protein gene product (PGP) 9.5, researchers can visualize and count IENFs. These fibers consist of unmyelinated C-fibers and thinly myelinated Aδ fibers. The ability to visualize and quantify IENFs from relatively noninvasive skin biopsies has significantly advanced research and clinical care for peripheral nerve diseases3.

In summary, assessing IENFs through skin biopsies provides valuable diagnostic and prognostic information, contributing to our understanding of peripheral neuropathies and potential treatment approaches1.

Learn more

1📷academic.oup.com2📷bing.com3📷practicalneurology.com4📷aappm.org5📷academic.oup.com6doi.org+2 more

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