How to compare the groups in which each group consists the data for two different duration?

10 November 2016 8,310 1 View

How can one collect the complete information of a plant's phytochemicals?

03 April 2015 210 10 View

How do you confirm the solubility apart from inspection?

01 February 2015 3,011 3 View

How can I prepare the Triton WR 1339 as 200mg/kg to induce hyperlipidemia?

01 February 2015 1,379 3 View

Which triton (x 100 or wr1339) might be used to induce hyperlipidemia?

In order to screen the hypolipidemic drugs, Triton induced hyperlipidemic animal model is being used. In most of the papers, Triton WR 1339 (tyloxapal) was used to induce hyperlipidemia. However,...

31 December 2014 3,237 3 View

What is the meaning of the word 'non caking' pertaining to paraffin wax, used in histology?

11 December 2014 7,362 9 View

Does any one experience with ideal diet induced hyperlipidemic animal model?

We need to test our herbal drug with diet induced hyperlipidemic animal model (Sprague Dawley rats) and we have gone through literature to know about high fat diet. There are many protocols...

11 December 2014 2,030 2 View

What parameters can be considered to confirm the perfect perfusion?

10 November 2014 6,373 9 View

Can anyone recommend a lyophilization facility in Chennai?

10 November 2014 7,957 5 View

Which is the best method for preparing plant extracts with polar, non-polar and mid-polar solvents?

I understand that there are several methods available to prepare plant extract namely maceration, decoction, infusion, Soxhlet Extraction, Microwave assisted extraction (MAE), supercritical fluid...

09 October 2014 745 9 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View