I need to execute the experiment on Triton WR 1339 induced hyperlipidemic rat model. The dose of Triton is 200mg/kg of body weight. I need some tips concerning this:
1. It is observed that many solutions are used as solvents for Triton WR 1339 such as saline solution (pH 7.4), Phosphate buffer (pH 7.2), 4% DMSO. Which solvent is best to dissolve the Triton WR 1339?
2. We purchased Triton WR 1339 from Sigma and it is in viscous liquid state (before i assume that, it is in powder form). So, if I want to prepare the Triton as 200mg / kg, how I can prepare?
3. Which is advisable either to prepare the Triton freshly on each time of experiment or prepare the stock and use it frequently. If preparing stock is ideal, how long it can be stored?
Trtiton WR 1339 comes as liquid. You could easily make a 10% solution of Triton in saline or PBS. You will need to inject 2ml of 10% solution per kg. This means that a 250gm rat will need 0.5ml solution. If you find 10% solution too viscous to work, reduce the concentration and increase the volume proportionately.
Triton is quite stable at room temperature. But since you will be using it for in vivo work, you should prepare your stock solution, filter sterilize, and save in aliquots you are likely to use in a single experiment to ensure sterility.
Trtiton WR 1339 comes as liquid. You could easily make a 10% solution of Triton in saline or PBS. You will need to inject 2ml of 10% solution per kg. This means that a 250gm rat will need 0.5ml solution. If you find 10% solution too viscous to work, reduce the concentration and increase the volume proportionately.
Triton is quite stable at room temperature. But since you will be using it for in vivo work, you should prepare your stock solution, filter sterilize, and save in aliquots you are likely to use in a single experiment to ensure sterility.
Dear Annaduria, For induction hyperlipidemia using Triton WR-1339, answer of your questins briefly are:
1. yes there are many solvents or solvent mixtures for dilution of WR-1339, but my suggestion for dilution of WR-1339 in order to animal injection is Normal saline (0.9% NaCl). of course as you will use it as i.p you have to consider sterile condition. As Tausif mentioned, I suggest you for preparing 10% (v:v) at first too. calculate 200mg/kg or 2ml/kg.
2. yes WR-1339 is a viscus liquid with flash point around 113 C. As I mentioned above you can prepare 10% v:v of WR-1339 in Normal saline (9 g/L). As handling of viscus liquid is not simple, it is better calculate equal weight of required volume base on its density. For this reason, viscus liquid sale/purchase is based on weight (g).
3. My suggestion is an stock preparation and aliqut as your each time total volume. Its solution is stable and do not worry about that, especially if you keep it in deep freeze.
best
mehdi
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