ali akbar Alizadeh

5 Questions 4 Answers 0 Followers

Questions related from ali akbar Alizadeh

Is L929 normal or cancer cell line?

Hi everyone. I know that the L929 has been originated from a normal cells, however, I am a bit suspicious about L929 cells available in ATCC whether it is normal or cancer cell line? I would...

01 January 2019 1,345 6 View

How to improve phage titre in M13 amplification?

I have infected E.coli TG1 by M13 ( Kanamycin resistant helper phage) and got colonies on TYE-kanamycin supplemented plates. Using one of these colonies I wanted to amplify and achieve the titre...

10 October 2013 1,437 8 View

What is the reason behind plaque formation?

I am confused about the origin of plaques in titering the M13 infected-bacteria mixed with LB-top agar though M13 phage is not the lytic one. Why should we see clear plaques with bacteria around...

09 September 2013 6,107 4 View

Can someone advise on the difference between colony and plaque?

For phage infected bacteria after plating, when should the "colony" term be used and when "plaque"? I do not know the exact difference.

09 September 2013 7,876 8 View

Can someone advise on how to see the right plaques of phage on LB plates?

I would like to know is it possible to see the phage (M13, kanamycin resistance) infected bacteria colonies on kanamycin supplemented LB plates?

07 July 2013 2,016 1 View