Does the THP-1 have this gene? If so, why don't you just knock-out the gene directly using CRISPR/Cas9 to study this gene's function?

I'm trying to understand point mutations of the gene with respect to the function of the gene. I already know what happens when we knock it out.

Thanks for response. Now I understand.

Atul,

Were you able to achieve CRISPR/Cas9 knock in in THP-1 cells in the end? I am also interested in doing this - any tips most appreciated!

Hi Michael,

No I still havent come across any such method. If i do , i will surely let you know !

Cheers,

Atul

I Atul and Michael, Have you been able to perform KI ThP-1 finally? I am very into it as well.... without success!

Thanks!