Evgeny, probably best to contact Qiagen directly to get a fast and accurate answer to your question. That being said, have you considered using Trizol/Tri-Reagent? You get both RNA and DNA (and protein if you are keen). 

Thank you, Ricardo! I tried Trizol, but it works no so good with small samples (300-1000 cells).

Hi Evgeny,

It is possible, I have used the following protocol. I don't think the DNA quality is as good as with a specific DNA extraction kit (I have only processed few samples), but better than nothing. As far as I know Trizol is very good for RNA, but not for DNA (e.g. hard to do Illumina DNAseq on samples extracted from Trizol).

This is the protocol:

Place gDNA elimination column on a washed S-Block, add 500µl AW1 Buffer (cat. No. 19081) to each well, seal with tape sheet and centrifuge at 6000rpm (5600g) for 2min.

Discard flow-through, add 500µl AW2 Buffer (cat. No. 19072) to each well, seal with tape sheet and centrifuge at 6000rpm (5600g) for 4min.

Discard flow-through, centrifuge gDNA elimination column for 4min at 6000rpm (5600g) with empty S-block to dry themembrane.

Place the columns on top of a 96-well master plate (Sarstedt, 72.1980.202): DNAplate_#_date

Add 50µl pre-warmed (~40°C) AE Buffer (cat. No. 19077) to the columns, incubate for approx. 30min, centrifuge at 6000rpm (5600g) for 4 min.

repeat step 5 to get 100µl coextracted DNA for rescuing some difficult genotyping samples.

Many thanks, Cristian, for the protocol! I will try it surely. But I think that DNA samples should be additionally treated with proteinase K to remove proteins with whom DNA can be bound.

Hi Evgeny,

this topic is very old, but do you remember the results?

did you isolate DNA from gDNA eliminator column?

best