Can someone advise on whether to express protein in 1x250 ml or 5x50 ml?

I want to express my protein (in E. coli) in 250 ml of culture medium (LB Broth). Is it better to culture my bacteria in one bottle (1 x 250 ml) or aliquot them in 5 separated bottles (5 x 50...

11 September 2013 5,656 1 View