Evgeny V. Denisov

11 Questions 35 Answers 0 Followers

Questions related from Evgeny V. Denisov

Does anybody know a solution (kit) for targeted bisulfite sequencing of a low number of cells?

Hi! We are planning to assess DNA methylation profile of 100-200 cells using bisulfite sequencing on the NextSeq500 instrument. However, the kits designed for single cells (Accel-NGS® Methyl-Seq...

23 January 2018 4,305 3 View

What are the markers to identify invasive front (cell migration) in breast tumors?

Dear colleagues, I would be grateful if you could suggest highly informative markers, which indicate tumor cell migration and invasive front in breast tumors. I found the following ones: CK14,...

30 March 2016 8,981 4 View

What distinguishing markers should I use to identify myofibroblasts in breast tumors?

Dear colleagues, I am going to identify myofibroblasts (CAFs) in breast tumors (FFPE samples) by confocal microscopy, but I am confused a little bit about what markers should I use? As I...

07 April 2015 9,321 3 View

Is it possible to get DNA from gDNA eliminator spin columns (Qiagen)?

Hi! I isolate tumor cells from breast tumor sections by laser microdissection and extract RNA by RNeasy Plus Micro Kit (Qiagen). But I would like to also get DNA from the same microdissected...

24 February 2015 3,043 5 View

Does anybody have any experience with aCGH on DNA after WGA?

We used cells isolated from tissue by laser microdissection to perform whole genome amplification (WGA) and then array comparative genome hybridization (aCGH). However, the results are no so good....

05 June 2014 6,345 2 View

Can anyone explain problems with Low Input Quick Amp Labeling in an agilent expression microarray?

Our group aimed to analyse gene expression in cells on different precancerous lesions using One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp Labeling, Agilent). For this...

03 April 2014 950 14 View

How to purify the isolated RNA if its concentration is very low?

We isolate the RNA from tumor cells which were obtained by laser microdissection, using RNeasy Micro Plus Kit (Qiagen). The concentration of the RNA varies from 7 to 9 ng/ul, RIN - 5.0-8.8,...

26 March 2014 9,391 12 View

Expression microarray: low input of RNA - can anyone help?

I am going to perform whole genome expression profiling of different tumor cells of one breast tumor. I isolate these tumor cells by laser microdissection and extract RNA. The total RNA...

28 October 2013 3,306 11 View

Why is there high expression of ABCB2 gene in breast tumor cells?

Our study demonstrates a high expression of ABCB2 gene in breast tumor cells. However, it is known that this gene is involved in antigen presentation. Could protein of this gene participates in...

15 April 2013 4,069 1 View

Whole transcriptome amplification from cells

Does anybody have experience in whole transcriptome amplification from cells? My usual study includes RNA extraction from cells (n=30-300), isolated from frozen tumors by laser-capture...

29 November 2012 6,508 3 View

Could chemotherapy influence on miRNA level?

If you have information demonstrating influence of chemotherapy on miRNA level, please share with me links. Thank you in advance!

14 September 2012 5,883 1 View