Evgeny V. Denisov

6 Questions 28 Answers 0 Followers

Questions related from Evgeny V. Denisov

Does anybody know a solution (kit) for targeted bisulfite sequencing of a low number of cells?

Hi! We are planning to assess DNA methylation profile of 100-200 cells using bisulfite sequencing on the NextSeq500 instrument. However, the kits designed for single cells (Accel-NGS® Methyl-Seq...

23 January 2018 4,231 3 View

Can anyone explain problems with Low Input Quick Amp Labeling in an agilent expression microarray?

Our group aimed to analyse gene expression in cells on different precancerous lesions using One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp Labeling, Agilent). For this...

03 April 2014 902 14 View

How to purify the isolated RNA if its concentration is very low?

We isolate the RNA from tumor cells which were obtained by laser microdissection, using RNeasy Micro Plus Kit (Qiagen). The concentration of the RNA varies from 7 to 9 ng/ul, RIN - 5.0-8.8,...

26 March 2014 9,351 12 View

Expression microarray: low input of RNA - can anyone help?

I am going to perform whole genome expression profiling of different tumor cells of one breast tumor. I isolate these tumor cells by laser microdissection and extract RNA. The total RNA...

28 October 2013 3,224 11 View

Whole transcriptome amplification from cells

Does anybody have experience in whole transcriptome amplification from cells? My usual study includes RNA extraction from cells (n=30-300), isolated from frozen tumors by laser-capture...

29 November 2012 6,440 3 View

Could chemotherapy influence on miRNA level?

If you have information demonstrating influence of chemotherapy on miRNA level, please share with me links. Thank you in advance!

14 September 2012 5,811 1 View