Dear colleagues,
We are measuring the diffusion rate of caffeine through rat skin using Franz cells. The problem we are facing is the huge data (caffeine concentration in receptor chamber) variability from cell to cell. Please see the Fig.
We use animals of approximately the same weight and sex.
Each skin patches was taken from the same abdominal area (to eliminate any individual anatomical differences). Skin patches were dissected and handled as carefully as possibly. Caffeine was added to donor chamber as 0.5 ml of caffeine 0.2% saline in PBS. t=37'C.
If we use an artificial skin (Strat-M) patches, we do not have this problem and diffusion rate in cells is way more uniform.
We are wondering what would be the cause of a such diffusion rate variability in rat skin patches and/or what we might be doing wrong?
Thank you all in advance
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