Which column from GenePix sheet correlates to the color in the cluster diagram?

25 February 2020 3,703 1 View

Is there a synthetic DNA modification that could prevent extension of the opposite strand?

For a Taq polymerase reaction, let's say that I have a primer A (which I cannot prevent from being extensible, it has a natural 3' end) which can hybridize to a known point on a synthetic DNA...

27 January 2020 9,085 3 View

Where in M&M 1913 article is it stated that [E]0 << [S]0?

I've been reading the Johnson & Goody translation of "Die Kinetik der Invertinwirkung" and I can't find the part where authors (M&M) actually claim that [E]0

15 March 2017 3,836 4 View

Can I analyze the results of this experiment with t test?

I have multiple parameters and want to examine their effect on the response variable, Y. The experimental design is as follows. There are 3 factors. Two of them: A1 and A2, have two levels (coded...

09 December 2016 4,440 16 View

Can I model a composite plate in a Nastran/Patran program so the result will give stress values at a specific thickness?

In modeling composite plates in a Nastran/Patran program using shell elements, results in the output file are for the mid-plane of each layer of composite. Does anybody know if it can be possible...

24 August 2014 1,725 6 View

How I can separate, purify, isolate BSA from DEX BSA?

I need protocol, if there is one, to separate BSA from compound DEX-BSA. I attached a chemical structure of DEX-BSA?? Any idea, protocol will be very helpfull.

21 August 2014 4,896 1 View

Can anyone explain the differences between programs for designing primers?

Although I am fully aware that different primer designing software are using different algorithms in their work, still they should give the similar primer pair combinations or not? Also, when some...

31 July 2014 2,648 1 View

Can anyone give me their advice on changing PCR settings for annealing and/concentration in Real Time Sybr green PCR?

I run real time sybr green pcr (rat hippocampus) and I came accros a primer dimer situation. I use 42.5 nM primers in final concentration. I am getting PDs in whole range of concentrations, from...

07 July 2014 3,890 3 View

What is the difference between heparin, citrate and EDTA tubes for blood collection and what is the effect on DNA?

Advantages/disadvantages, troubleshooting when doing real time PCR?

27 April 2014 8,274 9 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

How to find natural frequency of a bridge from vehicle induced vibrations?

i have frequencies of bridge that are effected by mass of vehicle. the problem is when any vehicle pass the bridge it would add the its mass to the bridge mass ultimately mass of the bridge will...

22 February 2021 10,004 3 View

No title

22 February 2021 9,932 11 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View