Milos Mitic

15 Questions 16 Answers 0 Followers

Questions related from Milos Mitic

Are there any studies that explore and compare the effect of esketamine and some classic AD in stressed mice?

How would one design the research study? If we try to compare them, when should we do behavioral tests?

13 November 2023 4,567 0 View

Behavioral tests in rodents

Immobility time in forced swim test could interpreted to be either to access depressive-like symptomatology or adaptation?

10 June 2017 1,786 4 View

Is injecting a destillaed water to animals i.p. aversive?

Can a distilled water be used as as vehicle for i.p. injections, when active substance is dissolved in dH20? I got some remarks that distilled water is hypotonic and that i.p. injections are...

22 December 2016 6,590 3 View

Connection between expression of 5ht1a and p11 gene upon stress and AD?

We know that p11 transports the 5-HT1B receptor from the cytoplasm to the membrane and increases density of this receptor on postsynaptic membrane and therefore upregulates serotonergic...

20 May 2016 4,765 0 View

Recovery time after estrous cycle check in female rats?

In investigation possible sex differences the important consideration taking into experiment female rats with proper distribution of estrous cycles. The wright way to estimate estrous cycle is...

23 March 2016 4,894 3 View

Protein concentration rat liver

How much to dilute whole cell liver tissue extract for micro Lowry protein concentration determination?

18 February 2016 1,775 3 View

How doI dissolve DEX-BSA for intracerebroventricular injections?

Is it just saline or for example CSF or is it combination of DMSO and CSF for instance? Anyone had a problem regarding the infusion of DEX-BSA?

19 October 2015 5,590 0 View

Anyone familiar with freezing behavior vs immobility behavior?

How can I distinct freezing behavior and immobility behavior? Namely, all of the software for quantification of freezing behavior counts immobility as freezing! Majority of papers has its own way...

20 May 2015 7,688 5 View

How I can separate, purify, isolate BSA from DEX BSA?

I need protocol, if there is one, to separate BSA from compound DEX-BSA. I attached a chemical structure of DEX-BSA?? Any idea, protocol will be very helpfull.

22 August 2014 5,175 1 View

Can anyone explain the differences between programs for designing primers?

Although I am fully aware that different primer designing software are using different algorithms in their work, still they should give the similar primer pair combinations or not? Also, when some...

01 August 2014 2,779 1 View

Can anyone give me their advice on changing PCR settings for annealing and/concentration in Real Time Sybr green PCR?

I run real time sybr green pcr (rat hippocampus) and I came accros a primer dimer situation. I use 42.5 nM primers in final concentration. I am getting PDs in whole range of concentrations, from...

08 July 2014 4,030 3 View

Does anyone have reviews about Fluoxetine effects on HPA axis?

Can anyone recommend a few reviews about fluoxetine usage on HPA axis deregulation in humans and in animal models of depression?

23 May 2014 9,761 2 View

What is the difference between heparin, citrate and EDTA tubes for blood collection and what is the effect on DNA?

Advantages/disadvantages, troubleshooting when doing real time PCR?

28 April 2014 8,603 9 View

How are GRE genes involved in neurogenesis?

Does anyone know of some gene candidates that have corticosteroid response elements (GRE) predominantly involved in stress-response and depression?

05 December 2013 1,996 3 View

5HIAA/5 HT turnover ratio in depression?

Can anyone explain me the meaning/use of 5HIAA/5 HT turnover ratio in depression? Why is this ratio important from the utilization of serotonin in the brain? In simple terms.

16 September 2013 3,686 10 View