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Questions related from Milos Mitic
How would one design the research study? If we try to compare them, when should we do behavioral tests?
13 November 2023 4,416 0 View
Can a distilled water be used as as vehicle for i.p. injections, when active substance is dissolved in dH20? I got some remarks that distilled water is hypotonic and that i.p. injections are...
22 December 2016 6,518 3 View
We know that p11 transports the 5-HT1B receptor from the cytoplasm to the membrane and increases density of this receptor on postsynaptic membrane and therefore upregulates serotonergic...
20 May 2016 4,709 0 View
In investigation possible sex differences the important consideration taking into experiment female rats with proper distribution of estrous cycles. The wright way to estimate estrous cycle is...
23 March 2016 4,822 3 View
How much to dilute whole cell liver tissue extract for micro Lowry protein concentration determination?
18 February 2016 1,711 3 View
Is it just saline or for example CSF or is it combination of DMSO and CSF for instance? Anyone had a problem regarding the infusion of DEX-BSA?
19 October 2015 5,528 0 View
I need protocol, if there is one, to separate BSA from compound DEX-BSA. I attached a chemical structure of DEX-BSA?? Any idea, protocol will be very helpfull.
22 August 2014 5,003 1 View
Although I am fully aware that different primer designing software are using different algorithms in their work, still they should give the similar primer pair combinations or not? Also, when some...
01 August 2014 2,710 1 View
I run real time sybr green pcr (rat hippocampus) and I came accros a primer dimer situation. I use 42.5 nM primers in final concentration. I am getting PDs in whole range of concentrations, from...
08 July 2014 3,957 3 View
Can anyone recommend a few reviews about fluoxetine usage on HPA axis deregulation in humans and in animal models of depression?
23 May 2014 9,700 2 View
Advantages/disadvantages, troubleshooting when doing real time PCR?
28 April 2014 8,425 9 View
Does anyone know of some gene candidates that have corticosteroid response elements (GRE) predominantly involved in stress-response and depression?
05 December 2013 1,934 3 View
Can anyone explain me the meaning/use of 5HIAA/5 HT turnover ratio in depression? Why is this ratio important from the utilization of serotonin in the brain? In simple terms.
16 September 2013 3,626 10 View