Are there any studies that explore and compare the effect of esketamine and some classic AD in stressed mice?

13 November 2023 0 5K Report

How would one design the research study? If we try to compare them, when should we do behavioral tests?

More Milos Mitic's questions See All

How to solve insufficient memory problem in VinaXB?

Hi y'all. When I try to dock a halogenated ligand with VinaXB.exe, I get the Setting up the scoring function ... Error: insufficient memory! message. When I use Vina.exe instead, I get results...

18 May 2021 6,287 3 View

Can you recelluralize a porcine lung scaffold with human cells?

I've read articles about deccelluralizing human lungs and recellularizing them with recipients' cells, or gene editing porcine lungs to be accepted by humans. But I couldn't find any where porcine...

14 October 2020 8,138 1 View

Which column from GenePix sheet correlates to the color in the cluster diagram?

Hey guys. I am reading a great article (Ross, D. T.; Perou, C. M. Dis. Markers 2001, 17, 99–109), and am trying to figure out the genetic footprints and differences between 2 tumor cell lines....

25 February 2020 3,843 1 View

Is there a synthetic DNA modification that could prevent extension of the opposite strand?

For a Taq polymerase reaction, let's say that I have a primer A (which I cannot prevent from being extensible, it has a natural 3' end) which can hybridize to a known point on a synthetic DNA...

27 January 2020 9,189 3 View

MALDI - TOF spectra of yeast, which software for classification use?

Dear all, Please, can anyone give me information, how to classify the MLADI-TOF (MS) yeast spectra without the manufacturer's software (Biotyper of Brucker)? Which software is most suitable for...

28 December 2017 9,479 3 View

Where in M&M 1913 article is it stated that [E]0 << [S]0?

I've been reading the Johnson & Goody translation of "Die Kinetik der Invertinwirkung" and I can't find the part where authors (M&M) actually claim that [E]0

15 March 2017 3,942 4 View

Can we tune metaheuristics using regression models?

Let's say that a metaheuristic algorithm has three parameters: A, B and C. Let Y denote its performance metric. For example, Y is the average difference between the objective function value of the...

22 December 2016 9,076 4 View

Is injecting a destillaed water to animals i.p. aversive?

Can a distilled water be used as as vehicle for i.p. injections, when active substance is dissolved in dH20? I got some remarks that distilled water is hypotonic and that i.p. injections are...

21 December 2016 6,548 3 View

How do you control seeds for generating random numbers and analyze their effect on metaheuristic algorithms?

Almost all metaheuristic algorithms (all that I know of) use randomly generated numbers to guide their search for the optimal solution. When you are evaluating a metaheuristic, do you set your own...

11 December 2016 6,779 10 View

Can I analyze the results of this experiment with t test?

I have multiple parameters and want to examine their effect on the response variable, Y. The experimental design is as follows. There are 3 factors. Two of them: A1 and A2, have two levels (coded...

09 December 2016 4,567 16 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What is the role of oxidative stress in toxin-induced carcinogenesis?

What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...

02 August 2024 3,174 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View