Which column from GenePix sheet correlates to the color in the cluster diagram?

25 February 2020 3,703 1 View

Is there a synthetic DNA modification that could prevent extension of the opposite strand?

For a Taq polymerase reaction, let's say that I have a primer A (which I cannot prevent from being extensible, it has a natural 3' end) which can hybridize to a known point on a synthetic DNA...

27 January 2020 9,085 3 View

Where in M&M 1913 article is it stated that [E]0 << [S]0?

I've been reading the Johnson & Goody translation of "Die Kinetik der Invertinwirkung" and I can't find the part where authors (M&M) actually claim that [E]0

15 March 2017 3,836 4 View

Can I analyze the results of this experiment with t test?

I have multiple parameters and want to examine their effect on the response variable, Y. The experimental design is as follows. There are 3 factors. Two of them: A1 and A2, have two levels (coded...

09 December 2016 4,440 16 View

Can I model a composite plate in a Nastran/Patran program so the result will give stress values at a specific thickness?

In modeling composite plates in a Nastran/Patran program using shell elements, results in the output file are for the mid-plane of each layer of composite. Does anybody know if it can be possible...

24 August 2014 1,725 6 View

How I can separate, purify, isolate BSA from DEX BSA?

I need protocol, if there is one, to separate BSA from compound DEX-BSA. I attached a chemical structure of DEX-BSA?? Any idea, protocol will be very helpfull.

21 August 2014 4,896 1 View

Can anyone explain the differences between programs for designing primers?

Although I am fully aware that different primer designing software are using different algorithms in their work, still they should give the similar primer pair combinations or not? Also, when some...

31 July 2014 2,648 1 View

Can anyone give me their advice on changing PCR settings for annealing and/concentration in Real Time Sybr green PCR?

I run real time sybr green pcr (rat hippocampus) and I came accros a primer dimer situation. I use 42.5 nM primers in final concentration. I am getting PDs in whole range of concentrations, from...

07 July 2014 3,890 3 View

What is the difference between heparin, citrate and EDTA tubes for blood collection and what is the effect on DNA?

Advantages/disadvantages, troubleshooting when doing real time PCR?

27 April 2014 8,274 9 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View