Which column from GenePix sheet correlates to the color in the cluster diagram?

25 February 2020 3,703 1 View

Is there a synthetic DNA modification that could prevent extension of the opposite strand?

For a Taq polymerase reaction, let's say that I have a primer A (which I cannot prevent from being extensible, it has a natural 3' end) which can hybridize to a known point on a synthetic DNA...

27 January 2020 9,085 3 View

Can I analyze the results of this experiment with t test?

I have multiple parameters and want to examine their effect on the response variable, Y. The experimental design is as follows. There are 3 factors. Two of them: A1 and A2, have two levels (coded...

09 December 2016 4,440 16 View

Can I model a composite plate in a Nastran/Patran program so the result will give stress values at a specific thickness?

In modeling composite plates in a Nastran/Patran program using shell elements, results in the output file are for the mid-plane of each layer of composite. Does anybody know if it can be possible...

24 August 2014 1,725 6 View

How I can separate, purify, isolate BSA from DEX BSA?

I need protocol, if there is one, to separate BSA from compound DEX-BSA. I attached a chemical structure of DEX-BSA?? Any idea, protocol will be very helpfull.

21 August 2014 4,896 1 View

Can anyone explain the differences between programs for designing primers?

Although I am fully aware that different primer designing software are using different algorithms in their work, still they should give the similar primer pair combinations or not? Also, when some...

31 July 2014 2,648 1 View

Can anyone give me their advice on changing PCR settings for annealing and/concentration in Real Time Sybr green PCR?

I run real time sybr green pcr (rat hippocampus) and I came accros a primer dimer situation. I use 42.5 nM primers in final concentration. I am getting PDs in whole range of concentrations, from...

07 July 2014 3,890 3 View

What is the difference between heparin, citrate and EDTA tubes for blood collection and what is the effect on DNA?

Advantages/disadvantages, troubleshooting when doing real time PCR?

27 April 2014 8,274 9 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

08 February 2021 2,793 2 View

What are the factors on which exchange current density of an electrode depends, and how can they be predicted?

While surface roughness is an obvious factor, this is not what is exactly asked. Rather, periodic variation of exchange current density (of an electrode) with solution chemistry (of electrolyte)...

30 January 2021 4,886 5 View

Solving diffusion- reversible chemical reaction equation: How to solve both terms numerically?

I am using finite difference method to discretize the parabolic equation (explicit method), but confused about the chemical reaction term. Solving the generic equation for i specie involves a...

24 January 2021 1,149 18 View

In Michaelis-Menten Kinetics, why K(subscript)On have units (mol^-1)(time^-1)?

where K(subscript)On is the rate of Enzyme (E) + Substrate (S) going to Enzyme-Substrate complex (ES). I think I understand why K(subscript)Off and K(subscript)catalysis have units of time^-1....

11 January 2021 649 8 View

Which antimicrobial test will be suitable to determine antimicrobial activity of silver nanoparticles?

There are some journal articles that reported well diffusion method to determine the antimicrobial activity of silver nanoparticles. Meanwhile, some reported disc diffusion method. Which method is...

06 January 2021 7,123 4 View