Who can suggest a fast positive control for beta-Gal staining ?

Hello, I want to stain MCF7 cells treated and untreated with beta- Gal. My treatment lasts for 12 days, then I harvest the cells. Does someone know how I could treat MCF7 cells to get a...

08 September 2018 9,266 10 View

How could I visualize senescence in vivo ?

I would like to see whether my in vivo treatment induces senescence in my tumor or the microenvironment......and was wondering if I could simply stain the surrounding skin (that has been around...

10 November 2017 3,767 3 View

Does Serum derived from the fetus contain hormones?

Hello all, I am working with hormone responsive cancer cells (MCF7, ER positive) and wondering whether I need to use stripped serum or not . In literature, some people either strip their...

09 October 2017 9,921 9 View

WHEN using WHICH enzyme mix to isolate tumor cells from xenograft masses ?

06 July 2017 6,201 2 View

Who's experience with the Comet assay, knows how to save costs especially for use of enzymes ( hOGG1,FPG or EndoIII)?

03 April 2017 4,449 4 View

How many B16 do you inject in mice and how long is incubation time before starting the experiment?

Hello, I've a general question as literature is inconsistant: Who has experience with melanoma in vivo models and can help me? I tried to inject 200 thousand cells s.c w/o Matrigel and started...

01 February 2017 6,377 8 View

Can I mix F2 and F3 generations in 1 big mouse Experiment?

Hello, I need to start a quite big mouse experiment and have right now F1 mice (parents were bought form a company) as breeding couples. They wont give enough mice though to start the experiment...

09 October 2016 7,970 4 View

Who looks at Estrogen Receptor alpha and/or Beta?

Hello, does anyone have a personal suggestion regarding Antibodies for Estrogen Receptor alpha and beta ? There are so many out there and usually I go for Cell Signalling, but the references do...

09 October 2016 5,205 2 View

Orally administered drug at ph 4 in Sodium Lactate. D or L- enantiomer?

I want to use Palbociclib in vivo and if in References is said how they diluted, they give you Lactate Buffer (50mM, pH4) as indication. 1) Do I have to care about DL isomers? 2) Could the pH...

07 August 2016 7,296 4 View

Who has experience with sub cutaneous injection of oil diluted drug in mice (NOD SCID)?

07 August 2016 5,265 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

What are the main conditions suited for synthesizing a PVDF thin film by solvent casting method?

I have been trying to synthesize PVDF thin film by solvent casting method. I started by using 2:3 DMSO: acetone as solvents. The solution was stirred for 3hrs at 60 deg and was dried at 90 degrees...

09 February 2021 8,850 3 View

H2O2 scavenging activity problem??

When I do the test of plant against the free radicals of H2O2 the control had a value about 0.9126 The critical point is when I prepared the extract with DMSO the absorbance appear more than 2...

09 February 2021 6,430 2 View