I would like to see whether my in vivo treatment induces senescence in my tumor or the microenvironment......and was wondering if I could simply stain the surrounding skin (that has been around the s.c. injected tumor ) for S. markers .
Most mature skin is going to be senescent (or at least react to the Beta-Gal stain the same) anyways. You might be able to engineer a reporter in your tumor that fluoresces when senescent proteins are generated that you could visualize with a UV bulb. Though that might be a lot more trouble than its worth.
-You could use some commercial kit for the staining of senescence associated beta-galactosidase like https://www.cellsignal.com/products/cellular-assay-kits/senescence-b-galactosidase-staining-kit/9860
You could use it to stain the tumour or the surrounding tissue.
Another option, if you have access to a In vivo imaging system, is to visualize in real-time the activity of beta-gal, you can look at this reference Article Real-Time Tracking and In Vivo Visualization of β-Galactosid...
Try SenTraGor - this is new commercial dye suitable for in vivo staining. This is modificated sudan black stain, which binds to lipofuscin, the marker of senescence.
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