Is it possible to do FACS on snap frozen cells ? They were isolated and directly dropped in liquid N2. They were not frozen with DMSO or FBS.
I have isolated granulosa cells from mice ovary using puncture method. Cells are healthy and growing in DMEM. Their morphology is same as granulosa cells. However I would like to use a molecular...
12 May 2024 3,889 3 View
It is from 7-hydroxy-4-methyl coumarin with HMTA in dil acetic acid at 90 degrees for 6 hrs and hydrolysed with 20% HCl, kept for 30 min. cooled overnight and extracted with ether.
15 April 2024 3,941 0 View
because people are citing/talking less about Classical texts discovered by Rishi/sages. Rishi has the broad scientific knowledge as per their practical and theoretical practices.
30 March 2024 2,013 0 View
I am working on T cell receptor repertoire analysis obtained from RNA seq data. I am trying to understand the CDR3 length and amino acid usage in my data set, where I have variable lengths of...
12 March 2024 2,999 0 View
is there any model or formula to calculate the length of the partial ground plane? thanks in advanced
19 January 2024 5,021 3 View
As there is globalisation and the digital revolution in every aspect of future learners who are so-called new millennium learners, how the Innovative Learning Environments fulfil the purpose....
15 January 2024 9,532 2 View
I am in the beginning phase of antenna design and i am confused regarding the shape of the radiator.Thanks in advanced
14 January 2024 8,760 3 View
The number of pores in the R.10 flow cell decreased significantly from 1500 to 500 after nanopore sequencing with only 24 samples multiplexing. I'm using the SQK-NBD114.24 kit as the standard for...
11 December 2023 7,407 0 View
scaps 1d IV Curve
16 November 2023 2,498 0 View
I am using photoshop for the same. Please suggest some good software for labelling SEM images like crater, holes etc
27 August 2023 3,877 3 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,047 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,241 2 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,568 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,012 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,727 6 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,976 3 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 761 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,724 2 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,747 4 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,142 4 View