18 Questions 39 Answers 0 Followers
Questions related from Vanessa S
Hello, I want to stain MCF7 cells treated and untreated with beta- Gal. My treatment lasts for 12 days, then I harvest the cells. Does someone know how I could treat MCF7 cells to get a...
09 September 2018 9,345 10 View
I would like to see whether my in vivo treatment induces senescence in my tumor or the microenvironment......and was wondering if I could simply stain the surrounding skin (that has been around...
11 November 2017 3,847 3 View
Hello all, I am working with hormone responsive cancer cells (MCF7, ER positive) and wondering whether I need to use stripped serum or not . In literature, some people either strip their...
10 October 2017 9,996 9 View
Hello all, I want to re-isolate MCF7 cells from xenograft masses and grow them in vitro (just a wee or two). In different protocols I red that people use always colagenases but sometimes also...
07 July 2017 6,304 2 View
Hello everybody, I want to try the comet assay, which is new for me. in order to see whether my treatments induce directly DNA strand breaks or via ROS formation I'd compare samples run with the...
04 April 2017 4,527 4 View
Hello, I've a general question as literature is inconsistant: Who has experience with melanoma in vivo models and can help me? I tried to inject 200 thousand cells s.c w/o Matrigel and started...
02 February 2017 6,480 8 View
Hello, does anyone have a personal suggestion regarding Antibodies for Estrogen Receptor alpha and beta ? There are so many out there and usually I go for Cell Signalling, but the references do...
10 October 2016 5,288 2 View
Hello, I need to start a quite big mouse experiment and have right now F1 mice (parents were bought form a company) as breeding couples. They wont give enough mice though to start the experiment...
10 October 2016 8,053 4 View
I want to use Palbociclib in vivo and if in References is said how they diluted, they give you Lactate Buffer (50mM, pH4) as indication. 1) Do I have to care about DL isomers? 2) Could the pH...
08 August 2016 7,398 4 View
Hello, I have some general questions/curiousities: I need to inject s.c. a drug that is first diluted in DMSO and than in oil (I use corn oil). Final DMSO conc is 5%. The total volume is 200µL,...
08 August 2016 5,356 2 View
Hello, I injected MCF7 cells in NOD SCID IL 2 RG null mice, after transplanting an estrogen releasing pellet (not between the shoulders on the back, but on the side). No matrigel, just cells...
08 August 2016 941 3 View
Hello everybody, I thought about getting a program in order to keep my library of publications under better control and being able to create a fast bibliography one day I might get there ;) Can...
05 May 2016 1,518 0 View
I want to look at the Estrogen Receptor (alpha and beta). Therefore I need an Ab that recognizes either N-term or C-term AND one that recognizes the central region. Can anybody suggest a good...
05 May 2016 1,519 0 View
Does anyone have a protocol to make your own estrogen free Serum ? I heard you can get the charcoal and stripp it your own, but can't find a protocol with refrence... And do you know what else is...
05 May 2016 8,517 1 View
I bought 10mg RNAse from Sigma, which comes in a drop of 50% Glycerol and Tris - HCl. I want to add 1mL solution to bring it to a nice working concentration of 10mg/mL. Now we discuss in the Lab...
02 February 2016 8,520 4 View
I heard that you should store DMSO not to long in a plastic falcon, but always take it fresh from the dark glass bottle. Is that true ? and if yes, does anybody have an explanation ? I can't find...
06 June 2015 2,854 9 View
For my experiments, where the medium composition is fundamental, I grow all cell lines in the same medium in order to keep comparable basic conditions. So far my choosen cell lines that have been...
05 May 2015 2,892 4 View
Does anybody know what is the highest concentration of a Stock solution of MTT (powder from Sigma ) in Culture Medium ? I know from Sigma that they tested their powder up to 5mg/ mL and that in...
03 March 2015 6,269 4 View
