Is it crucial to use ATCC recomended medium to expand cell lines upon arrival?

03 April 2018 6,390 4 View

How can i get rid of antibiotic (Penicillin/Streptomycin) tolerant bacteria from a cancer cell line?

I was trying to expand a cell line provided from a colleague but i noticed more than normal cell death in the culture just after the cells where thawed and attached to a t25 flask. Since then, the...

31 December 2017 2,124 8 View

What to consider before starting to develop drug-resistant cancer cell line models?

Hi. I want to start experiments in a few months, but i want to cover every single detail before starting with such time consuming protocols. To give a little bit of context, i aim to develop...

11 December 2017 3,832 4 View

Which are some DHT alternatives for IC50 assays in breast cancer cell lines?

10 November 2017 4,592 0 View

Why would the color of the DMEM F12 medium seem to be diluted?

The normal color of the media after being prepared with water, sodium bicarbonate and FBS should be an intense red color (as far as i know), but this time the color of the media is still red but...

09 October 2017 3,197 0 View

R1881 or DHT for AR receptor stimulation?

09 October 2017 8,640 0 View

Effect of quiescent/senescent cells on formazan production in MTT assays?

I was wondering if quiescent cells will have enough metabolic activity to interfere with the interpretation of the results (% of growth inhibition), in other words, if the formazan production...

07 August 2017 9,875 0 View

Will these changes affect the behaviour of the MCF7 cell line in future passages?

07 August 2017 5,412 6 View

Colorimetric assay for determining cancer cell growth inhibition?

07 August 2017 6,745 4 View

Could RPMI-1640 be replaced with DMEM to grow HCC1954?

05 June 2017 8,853 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View