15 Questions 25 Answers 0 Followers
Questions related from Mauricio de la Rosa
I ordered some breast cancer cell lines from ATCC (CAMA-1, SKBR3, MDAMB453), but i don´t have the media that atcc recomend to culture those cell lines (EMEM, McCoy's 5a Medium Modified and L15,...
04 April 2018 6,484 4 View
I was trying to expand a cell line provided from a colleague but i noticed more than normal cell death in the culture just after the cells where thawed and attached to a t25 flask. Since then, the...
01 January 2018 2,266 8 View
I´ve seen that some researchers use DHT (Dihydrotestosterone AKA 5α-Androstan-17β-ol-3-one) when performing assays involving antiandrogens while others don´t. Is it really needed or does it...
01 January 2018 9,428 2 View
Hi. I want to start experiments in a few months, but i want to cover every single detail before starting with such time consuming protocols. To give a little bit of context, i aim to develop...
12 December 2017 3,926 4 View
Hi. I´m about to start IC50 assays in breast cancer cell lines using antiandrogens and the only thing that stops me from starting is that i don´t have DHT yet. It seems it will be quite difficult...
11 November 2017 4,678 0 View
I´m having trouble getting DHT, something country-provider related, so i was asking my self if i could use R1881 instead of DHT in order to activate the androgen receptor in cancer cell lines....
10 October 2017 8,778 0 View
The normal color of the media after being prepared with water, sodium bicarbonate and FBS should be an intense red color (as far as i know), but this time the color of the media is still red but...
10 October 2017 3,285 0 View
I was wondering if quiescent cells will have enough metabolic activity to interfere with the interpretation of the results (% of growth inhibition), in other words, if the formazan production...
08 August 2017 9,980 0 View
Hi everyone.I´m new to cell culture and i was wondering if this morphology (image 1) is common when growing MCF7 cells and if clustered cells (image 2) give rise to changes in the cell line...
08 August 2017 5,504 6 View
Hi Does anyone knows a colorimetric (or relatively easy) assay to specifically determine growth inhibition?I´ve seen that some persons use MTS, crsystal violet, tripan blue, etc. But this assays...
08 August 2017 6,834 4 View
So far i haven´t found a single paper in which they grow the breast cancer cell line HCC1954 in other medium besides RPMI-1640. I wish to know if any of you have already try growing this cell line...
06 June 2017 8,952 2 View
Hi everyone.Does anyone knows a radiation-free method for glucose uptake in adipose tissue from rats or mice?
05 May 2017 1,545 4 View
With the help of researchgate´s community I found that to determine if a cell line is clinically-relevant resistant there needs to be a 2 - 5 fold change increase in the IC50 values of the cell...
03 March 2017 8,290 5 View
I´m struggling with deciding which media to use (I´m new in this cell culture field). Rigth now i have MEM and DMEM media on the lab, but i´ve seen that in some articles they use specific media...
01 January 1970 2,806 5 View
Reading many papers I´ve found that in this context (cell proliferation) using metabolic assays such as MTT is like "the gold standard". Many papers use this approach when trying to determine...
01 January 1970 3,026 4 View
