We were in verve of studying bacterial cell adhesion studies on various cell lines. We wanted to target Caco2 cell line as it is colon cancer cell line. Is it really suitable for this study>?
Remember that Caco2 cells can be cultured in confluence for extended time in order to make the polarization of hte cells, and thus making the apical surface of the cells more likely to represent the tissue surface... you could also use subconlfuent cells considering that polarization is not the same.
I worked with attenauated Salmonella on several epithelial cells, but did not try the CaCo2 model, but i know it was being used in my two previous labs.... i might be able to found someting useful for you
That is awesome ! We were also just looking out for some source Dr. Martin. Can we in anyways obtain the cell line?
Yes, some researchers have successfully used this and other cell lines. Our Lab has done some work in the area of probiotic cell adhesion (Microbiology Research Lab, Life science) and we are still doing more. You may want to contact us to share more info# Rohit
@kayoed: That would be awesome. I would love to. We can actually collaborate. We have some amazing ideas lined up.
Dear Rohit, Caco-2 cells are most explored for checking bacterial adhesion. You may find plenty of literature over it. You can go ahead without any second thought in mind.
Dear Rohit, Caco-2 cells are suitable for checking bacterial adhesion including also probiotic strains. I can recommend it also to you.
There is also a Caco2-bbe1 cell line which which was cloned from the Caco2 cell line. Caco2-bbe1 can be grown as polarized cells and when grown in transwells offer both apical and basolateral surfaces (more comparable to the human colon).
Dear Rohit, Caco-2 cells are suitable for checking bacterial adhesion including also probiotic strains. I can recommend it also to you.
Dear Rohit, we have successfully used: https://www.researchgate.net/publication/250006470_Adhesion_of_lactic_acid_bacteria_to_Caco-2_cells_-_Evaluation_of_different_detection_methods?ev=prf_pub
Article Adhesion of lactic acid bacteria to Caco-2 cells — Evaluatio...
I can see very interesting publication of prof. Péter Gálfi and his team. Igor Styriak
Dear Rohit,
The Caco-2 are suitable for checking bacterial adhesion including probiotics. But, you must be aware that they not produce mucus, therefore this in vitro system is not fully realistic. In relation of your research you should decide which is the cell line that better fits in your study. If you do not mind, I would like to advice the attached paper.
All the best with your research,
Roberto.
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