Double staining protocol for immuno fluorescence?

I am interested to check two antibodies on colon tissue (paraffin embedded) which I strongly believe show co-localization. What is the simple and best protocol ?

06 July 2016 7,733 7 View

Can colonic goblet cells be cultured?

Organoid culture is now well established method of growing goblet cells. It was said that these mucous secretory cells after isolation from the tissue die when put on culture plate. So is there...

04 May 2016 9,565 4 View

Is Caco2 cell line suitable for adhesion assay for probiotic strains ?

We were in verve of studying bacterial cell adhesion studies on various cell lines. We wanted to target Caco2 cell line as it is colon cancer cell line. Is it really suitable for this study>?

04 May 2016 6,639 13 View

How do we convert IC50 from cell culture to tissue explants?

Ho can we calculate IC50 (10nm)of Cytokine inhibitor in mouse macrophage cells into tissue explants of colon and lung?

02 March 2016 5,426 0 View

What is the attachment substance for culturing intestinal globlet cells?

Like we culture endothelial cells in fibronectin coated flasks, what would be the ideal adherent used in culturing isolated globlet cells from mice?

08 September 2015 2,367 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View