10nm of drug to be added?

If suppose the drug concentration is 10nM which is to be added, how do we calculate the amount to be added as final volume in 1mL of cell medium?

07 August 2016 2,565 2 View

Double staining protocol for immuno fluorescence?

I am interested to check two antibodies on colon tissue (paraffin embedded) which I strongly believe show co-localization. What is the simple and best protocol ?

06 July 2016 7,733 7 View

Can colonic goblet cells be cultured?

Organoid culture is now well established method of growing goblet cells. It was said that these mucous secretory cells after isolation from the tissue die when put on culture plate. So is there...

04 May 2016 9,565 4 View

Is Caco2 cell line suitable for adhesion assay for probiotic strains ?

We were in verve of studying bacterial cell adhesion studies on various cell lines. We wanted to target Caco2 cell line as it is colon cancer cell line. Is it really suitable for this study>?

04 May 2016 6,639 13 View

What is the attachment substance for culturing intestinal globlet cells?

Like we culture endothelial cells in fibronectin coated flasks, what would be the ideal adherent used in culturing isolated globlet cells from mice?

08 September 2015 2,367 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View