Ho can we calculate IC50 (10nm)of Cytokine inhibitor in mouse macrophage cells into tissue explants of colon and lung?
If suppose the drug concentration is 10nM which is to be added, how do we calculate the amount to be added as final volume in 1mL of cell medium?
07 August 2016 2,663 2 View
I am interested to check two antibodies on colon tissue (paraffin embedded) which I strongly believe show co-localization. What is the simple and best protocol ?
06 July 2016 7,794 7 View
Organoid culture is now well established method of growing goblet cells. It was said that these mucous secretory cells after isolation from the tissue die when put on culture plate. So is there...
04 May 2016 9,622 4 View
We were in verve of studying bacterial cell adhesion studies on various cell lines. We wanted to target Caco2 cell line as it is colon cancer cell line. Is it really suitable for this study>?
04 May 2016 6,703 13 View
Like we culture endothelial cells in fibronectin coated flasks, what would be the ideal adherent used in culturing isolated globlet cells from mice?
08 September 2015 2,441 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.
06 August 2024 8,848 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View