Rohit Gundamaraju

6 Questions 17 Answers 0 Followers

Questions related from Rohit Gundamaraju

10nm of drug to be added?

If suppose the drug concentration is 10nM which is to be added, how do we calculate the amount to be added as final volume in 1mL of cell medium?

08 August 2016 2,603 2 View

Double staining protocol for immuno fluorescence?

I am interested to check two antibodies on colon tissue (paraffin embedded) which I strongly believe show co-localization. What is the simple and best protocol ?

07 July 2016 7,771 7 View

Can colonic goblet cells be cultured?

Organoid culture is now well established method of growing goblet cells. It was said that these mucous secretory cells after isolation from the tissue die when put on culture plate. So is there...

05 May 2016 9,599 4 View

Is Caco2 cell line suitable for adhesion assay for probiotic strains ?

We were in verve of studying bacterial cell adhesion studies on various cell lines. We wanted to target Caco2 cell line as it is colon cancer cell line. Is it really suitable for this study>?

05 May 2016 6,680 13 View

How do we convert IC50 from cell culture to tissue explants?

Ho can we calculate IC50 (10nm)of Cytokine inhibitor in mouse macrophage cells into tissue explants of colon and lung?

03 March 2016 5,479 0 View

What is the attachment substance for culturing intestinal globlet cells?

Like we culture endothelial cells in fibronectin coated flasks, what would be the ideal adherent used in culturing isolated globlet cells from mice?

09 September 2015 2,406 1 View