I am performing an experiment in CHO cells with or without a chloride/bicarbonate exchanger protein expressed in the membrane.
When I replace normal buffer containing 130 mM chloride (pH 7.4) with 0 chloride, 50 mM bicarbonate buffer (also pH 7.4), the intracellular pH goes down in the control cells not expressing the exchanger.
What is the reason for this decrease?
The cells expressing the exchanger show an increase in intracellular pH, which is expected due to chloride/bicarbonate exchange.
It may be due to the nonbicarbonate buffers in the media, have a look here Crit Care Med. 2001 May;29(5):1033-9.
Initial effect of sodium bicarbonate on intracellular pH depends on the extracellular nonbicarbonate buffering capacity.
Levraut J1, Giunti C, Ciebiera JP, de Sousa G, Ramhani R, Payan P, Grimaud D.
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The helpfulness of your answer is more than I could have ever expected! Thank you a hundred times!
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